斑马鱼 NAD(P)H：醌氧化还原酶 1(nqo1)在成年器官和胚胎中的表达。
The expression of zebrafish NAD(P)H:quinone oxidoreductase 1(nqo1) in adult organs and embryos.
机构信息
Development and Regeneration Key Laboratory of Sichuan Province, Department of Anatomy and Histology and Embryology, School of Basic Medical Sciences, Chengdu Medical College, Chengdu, Sichuan, 610500, China.
School of Biomedical Sciences, Chengdu Medical College, Chengdu, Sichuan, 610500, China.
出版信息
Gene Expr Patterns. 2020 Dec;38:119134. doi: 10.1016/j.gep.2020.119134. Epub 2020 Sep 1.
NQO1, NAD(P)H: quinone oxidoreductase 1, was first identified in rat and its role has been extensively studied. Even the roles of NQO1 in the maintenance of physiological function and disease were largely addressed, whether the tissue specific functions of the NQO1 in organ development remains unknown. In the current study, we identified two NQO1 isoforms (isoform 1 and isoform 2) and examined the expression of nqo1 variants in adult zebrafish organs and embryos at different stages. In adult organs, RT-PCR result indicated that nqo1 variant 1 was mainly expressed in stomach and intestine, while nqo1 variant 2 was expressed in all organs investigated except for heart. Further, RT-PCR result showed that the nqo1 variant 1 and variant 2 were expressed at all the embryonic stages, but nqo1 variant 1 expression level was much lower than that of nqo1 variant 2. To specifically examine the expression pattern of these two different nqo1 variants, we did whole mount in situ hybridization and the results demonstrated that, both of them were maternally expressed at 8-cell stage, and they were all expressed ubiquitously at early stage. At 24 hpf, nqo1 variant 2 was mainly expressed in yolk cells, and slightly in head and eyes. At 48 hpf, nqo1 variant 2 was restricted in lateral line neuromasts. From 72 hpf to 144 hpf, nqo1 variant 2 was mainly restricted in branchial arch, liver, swimming bladder and lateral line neuromasts, while from 124 hpf to 192 hpf, nqo1 variant 2 only restricted in liver, and disappeared in lateral line neuromasts. On the contrary, at the late embryonic stage, nqo1 variant 1 was only expressed in liver and swimming bladder while not in branchial arch and lateral line neuromasts. In conclusion, we systematically analyzed the expression pattern of nqo1 variant 1 and variant 2 in zebrafish at different embryonic stages, and our data implied the possible role of nqo1 in regulating liver, branchial arch and lateral neuromasts development.
NQO1，即 NAD(P)H：醌氧化还原酶 1，最初在大鼠中被发现，其作用已得到广泛研究。尽管 NQO1 在维持生理功能和疾病中的作用已被广泛研究，但 NQO1 在器官发育中的组织特异性功能是否仍然未知。在本研究中，我们鉴定了两种 NQO1 同工型（同工型 1 和同工型 2），并检查了 nqo1 变体在成年斑马鱼器官和不同发育阶段胚胎中的表达。在成年器官中，RT-PCR 结果表明，nqo1 变体 1 主要在胃和肠道中表达，而 nqo1 变体 2 则在除心脏以外的所有研究器官中表达。此外，RT-PCR 结果表明，nqo1 变体 1 和变体 2 均在所有胚胎阶段表达，但 nqo1 变体 1 的表达水平远低于 nqo1 变体 2。为了专门检查这两种不同 nqo1 变体的表达模式，我们进行了全胚胎原位杂交，结果表明，它们在 8 细胞期均呈母体表达，在早期均呈广泛表达。在 24 hpf 时，nqo1 变体 2 主要在卵黄细胞中表达，在头部和眼睛中略有表达。在 48 hpf 时，nqo1 变体 2 局限于侧线神经丘。从 72 hpf 到 144 hpf，nqo1 变体 2 主要局限于鳃弓、肝脏、鳔和侧线神经丘，而从 124 hpf 到 192 hpf，nqo1 变体 2 仅局限于肝脏，而在侧线神经丘中消失。相反，在胚胎晚期，nqo1 变体 1 仅在肝脏和鳔中表达，而不在鳃弓和侧线神经丘中表达。总之，我们系统地分析了 nqo1 变体 1 和变体 2 在不同胚胎阶段斑马鱼中的表达模式，我们的数据表明 nqo1 可能在调节肝脏、鳃弓和侧线神经丘发育中发挥作用。
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