College of Chemistry and Life Science, Zhejiang Normal University, Jinhua 321004, China.
J Environ Sci (China). 2009;21(5):691-5. doi: 10.1016/s1001-0742(08)62325-6.
To provide basic toxicity data for formulating risk characterization benchmarks, the effects of lead on survival, locomotion, and sperm morphology were investigated in the Asian earthworm Pheretima guillelmi. The LC50 of P. guillelmi for 7 and 14 d were 4285 +/- 339 mg/kg and 3207 +/- 248 mg/kg, which shows P. guillelmi can tolerate a higher concentration of lead nitrate. The average weight of the surviving earthworms decreased at concentration of 2800 mg Pb/kg soil, and the locomotor ability of earthworms exposed to a range of soil Pb concentrations showed a general decrease with increasing Pb concentrations. We also presented data depicting the sperm morphology of earthworms, which shows potential as a sensitive biomarker for measuring the effects of heavy metal on reproduction.
为了提供制定风险特征基准的基本毒性数据，本研究调查了铅对赤子爱胜蚓（Pheretima guillelmi）生存、运动和精子形态的影响。赤子爱胜蚓的 7 天和 14 天 LC50 值分别为 4285±339mg/kg 和 3207±248mg/kg，表明赤子爱胜蚓可以耐受更高浓度的硝酸铅。在 2800mg Pb/kg 土壤浓度下，幸存蚯蚓的平均体重下降，暴露于不同土壤铅浓度的蚯蚓的运动能力普遍随着铅浓度的增加而下降。我们还提供了蚯蚓精子形态的数据，这表明它可能是一种敏感的生物标志物，可用于测量重金属对生殖的影响。
