夹心培养肝细胞：评估基于肝胆转运体的药物相互作用和肝毒性的体外模型。
Sandwich-cultured hepatocytes: an in vitro model to evaluate hepatobiliary transporter-based drug interactions and hepatotoxicity.
机构信息
University of North Carolina at Chapel Hill, 27599-7569, USA.
出版信息
Drug Metab Rev. 2010 Aug;42(3):446-71. doi: 10.3109/03602530903491881.
Abstract
Sandwich-cultured hepatocytes (SCH) are a powerful in vitro tool that can be utilized to study hepatobiliary drug transport, species differences in drug transport, transport protein regulation, drug-drug interactions, and hepatotoxicity. This review provides an up-to-date summary of the SCH model, including a brief history of, and introduction to, the use of SCH, as well as methodology to evaluate hepatobiliary drug disposition. A summary of the literature that has utilized this model to examine the interplay between drug-metabolizing enzymes and transport proteins, drug-drug interactions at the transport level, and hepatotoxicity as a result of altered hepatic transport also is provided.
摘要
夹层培养肝细胞（SCH）是一种强大的体外工具，可用于研究肝胆药物转运、药物转运的种属差异、转运蛋白调节、药物相互作用和肝毒性。本文综述了 SCH 模型的最新研究进展，包括 SCH 的简要历史和应用介绍，以及评估肝胆药物处置的方法学。本文还综述了利用该模型研究药物代谢酶和转运蛋白相互作用、转运水平的药物相互作用以及改变肝脏转运导致肝毒性的文献。
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