Dich J, Grunnet N
The Panum Institute, University of Copenhagen, Copenhagen, Denmark.
Methods Mol Biol. 1990;5:161-76. doi: 10.1385/0-89603-150-0:161.
With the advent in 1969 of the collagenase-perfusion technique for the high-yield preparation of isolated, differentiated hepatocytes (1), easy establishment of primary cultures of hepatocytes was made possible. Since then, this experimental system has been increasingly used in many research fields.