Dich J, Grunnet N
The Panum Institute, University of Copenhagen, Copenhagen, Denmark.
Methods Mol Biol. 1990;5:161-76. doi: 10.1385/0-89603-150-0:161.
With the advent in 1969 of the collagenase-perfusion technique for the high-yield preparation of isolated, differentiated hepatocytes (1), easy establishment of primary cultures of hepatocytes was made possible. Since then, this experimental system has been increasingly used in many research fields.
随着1969年胶原酶灌注技术的出现,该技术可用于高产率制备分离的、分化的肝细胞(1),肝细胞原代培养得以轻松建立。从那时起,这个实验系统在许多研究领域中得到了越来越多的应用。