Department of Otorhinolaryngology-Head and Neck Surgery, China-Japan Union Hospital, Bethune Faculty of Medicine, Jilin University, Changchun, China.
Am J Rhinol Allergy. 2010 Jan-Feb;24(1):e23-8. doi: 10.2500/ajra.2010.24.3414.
Minimal persistent inflammation (MPI) is considered another piece of the complex puzzle of allergic inflammation. Although some studies regarding MPI have been reported, no study has evaluated the effects of MPI on the structure changes at the site of allergic reaction. This study investigates whether long-time MPI during allergic rhinitis (AR) results in some features of tissue remodeling in the nasal mucosa.
An animal model of MPI was developed by repeated nasal challenge with low concentration of ovalbumin (OVA) in sensitized guinea pigs. The models were assessed by allergic symptom after antigen challenge, eosinophil infiltration in the nasal mucosa, and intercellular adhesion molecule (ICAM) 1 expression on nasal epithelial cells. The histopathological changes in nasal mucosa were determined by Alcian blue-periodic acid-Schiff and Masson's trichrome staining. The expression of transforming growth factor (TGF) beta(1) and matrix metalloproteinase (MMP) 9 was examined by immunofluorescence under a confocal laser scan microscope.
When sensitized animals were challenged with the low concentration of 0.01% OVA, the symptom of sneezing disappeared, but there were still mild eosinophils infiltration and weak ICAM-1 expression, which indicated the success of MPI models. Moreover, the number of goblet cells and the percentage area of collagen deposition were both mildly increased. The expression of MMP-9 and TGF-beta(1) was also weakly elevated.
We have successfully established MPI models and proved long-time MPI may result in mild features of remodeling in the nasal mucosa, which provide new insights into the unexpected potential effects of MPI on the structural changes.
微量持续性炎症（MPI）被认为是过敏炎症这一复杂谜题的另一个组成部分。虽然已经有一些关于 MPI 的研究报告，但没有研究评估 MPI 对过敏反应部位结构变化的影响。本研究旨在探讨变应性鼻炎（AR）期间的长时间 MPI 是否会导致鼻黏膜组织重塑的某些特征。
通过在致敏豚鼠的鼻腔中反复用低浓度卵清蛋白（OVA）进行挑战，建立 MPI 动物模型。通过抗原挑战后的过敏症状、鼻黏膜嗜酸性粒细胞浸润和鼻上皮细胞细胞间黏附分子（ICAM）1 表达来评估模型。通过阿利新蓝-过碘酸希夫和 Masson 三色染色来确定鼻黏膜的组织学变化。通过共聚焦激光扫描显微镜下的免疫荧光检查转化生长因子（TGF）β1 和基质金属蛋白酶（MMP）9 的表达。
当致敏动物用 0.01% OVA 的低浓度进行挑战时，打喷嚏的症状消失，但仍有轻度嗜酸性粒细胞浸润和弱的 ICAM-1 表达，这表明 MPI 模型成功建立。此外，杯状细胞数量和胶原沉积面积百分比均轻度增加。MMP-9 和 TGF-β1 的表达也略有升高。
我们成功建立了 MPI 模型，并证明长时间的 MPI 可能导致鼻黏膜轻度重塑特征，这为 MPI 对结构变化的意外潜在影响提供了新的见解。
