Cheng Zhao-Dong, Hu Shi-Lian, Sun Yu-Bei, Xu Wei-Ping, Shen Gan, Kong Xiang-Yong
Department of Gerontology, Province Hospital of Anhui Medical University, Hefei, Anhui 230001, PR China.
Chin J Cancer. 2010 Feb;29(2):163-6. doi: 10.5732/cjc.009.10305.
Transcriptional silencing induced by CpG island methylation is believed to be one of the important mechanisms of carcinogenesis. Checkpoint with fork head-associated and ring finger (CHFR) governs the transition from prophase to prometaphase in response to mitotic stress. This study was to analyze the relationship between the methylation of CHFR gene and the clinicopathologic features of gastric cancer, and the difference of results between methylation-specific polymerase chain reaction (MSP) and combined bisulfite restriction analysis (COBRA) in detecting aberrant methylation of CHFR gene in gastric cancer.
Both MSP and COBRA methods were used to detect the promoter methylation of CHFR gene in gastric cancer specimens from 64 patients. The relationship between methylation status of CHFR gene and the clinicopathologic features of gastric cancer were analyzed using SPSS16.0.
The methylation rates of CHFR gene promoter were significantly higher in gastric cancer samples than in the corresponding paracancer normal gastric mucosa by MSP (51.6% vs. 18.8%, P < 0.001). However, there was no significant correlation between methylation status of CHFR gene and the clinicopathologic parameters of gastric cancer, including age, gender, tumor size, clinical stage, Borrman type, tumor invasion depth, differentiation, and lymph node metastasis (P > 0.05). Aberrant methylation of the CHFR gene was detected in 27 (42.2%) of the 64 specimens of gastric cancer using COBRA, which did not significantly differ from that using MSP (P > 0.05).
Aberrant methylation of the CHFR gene is a frequent event in the carcinogenesis of gastric cancer. Detecting the methylation of CHFR gene in gastric mucosa may conduce to the diagnosis of gastric cancer. No difference was found between MSP and COBRA in detecting promoter methylation of CHFR gene in gastric cancer.
CpG岛甲基化诱导的转录沉默被认为是致癌的重要机制之一。含叉头相关和环指结构域的检查点蛋白（CHFR）可响应有丝分裂应激调控从前期到前中期的转变。本研究旨在分析CHFR基因甲基化与胃癌临床病理特征之间的关系，以及甲基化特异性聚合酶链反应（MSP）和联合亚硫酸氢盐限制分析（COBRA）在检测胃癌中CHFR基因异常甲基化时结果的差异。
采用MSP和COBRA方法检测64例胃癌患者标本中CHFR基因启动子甲基化情况。运用SPSS16.0分析CHFR基因甲基化状态与胃癌临床病理特征之间的关系。
通过MSP检测发现，胃癌样本中CHFR基因启动子的甲基化率显著高于相应的癌旁正常胃黏膜（51.6%对18.8%，P<0.001）。然而，CHFR基因甲基化状态与胃癌的临床病理参数，包括年龄、性别、肿瘤大小、临床分期、Borrmann分型、肿瘤浸润深度、分化程度及淋巴结转移之间无显著相关性（P>0.05）。使用COBRA在64例胃癌标本中的27例（42.2%）检测到CHFR基因异常甲基化，与MSP检测结果无显著差异（P>0.05）。
CHFR基因异常甲基化在胃癌发生过程中是常见事件。检测胃黏膜中CHFR基因甲基化可能有助于胃癌诊断。在检测胃癌中CHFR基因启动子甲基化方面，MSP和COBRA未发现差异。
