Institute for Biological Sciences, National Research Council, Ottawa, ON, Canada K1A 0R6.
Carbohydr Res. 2010 Mar 30;345(5):644-8. doi: 10.1016/j.carres.2009.11.026. Epub 2009 Dec 16.
The structure of the antigenic O-polysaccharide (O-PS) produced by Escherichia coli serotype O:70 was determined by analysis of the chromatographically purified O-PS polymer prepared by mild hydrolysis of its aqueous phenol-extracted smooth-type somatic lipopolysaccharide. The O-PS is composed of D-glucose, D-galactose, D-fucose, 2-acetamido-2-deoxy-D-galactose, and 3-acetamido-3-deoxy-D-quinovose in a ratio of 1:1:1:1:1. From the use of DOC-PAGE, methylation, Smith-type periodate oxidation, and (1)H and (13)C NMR spectroscopy, including 2D experiments, the O-PS was shown to be a polymer of a branched repeating pentasaccharide unit having the structure: [structure: see the text]
大肠杆菌血清型 O:70 产生的抗原 O-多糖（O-PS）的结构通过温和水解其水相酚提取的光滑型体脂多糖制备的色谱纯 O-PS 聚合物的分析来确定。O-PS 由 D-葡萄糖、D-半乳糖、D-岩藻糖、2-乙酰氨基-2-脱氧-D-半乳糖和 3-乙酰氨基-3-脱氧-D-奎诺糖以 1:1:1:1:1 的比例组成。使用 DOC-PAGE、甲基化、Smith 型高碘酸盐氧化和（1）H 和（13）C NMR 光谱，包括 2D 实验，表明 O-PS 是具有以下结构的支化重复五糖单元的聚合物：[结构：见正文]
