Institute for Medical Microbiology and Virology, University Medical Center Eppendorf, Martinistrasse 52, 20246 Hamburg, Germany.
Institut de Génétique Moléculaire de Montpellier, CNRS UMR 5535, 34293 Montpellier Cedex 5, France.
Virology. 2010 Mar 30;399(1):23-30. doi: 10.1016/j.virol.2009.12.028. Epub 2010 Jan 27.
Recently, the first human infection with an exogenous gammaretrovirus (XMRV) was reported. In its initial description, XMRV was confined to prostate stromal fibroblasts, although subsequent reports demonstrated XMRV protein expression in prostate epithelial cells. Most recently, XMRV has been detected in blood cells of patients with chronic fatigue syndrome. The aim of this study was to elucidate the transmission routes and tissue tropism of XMRV by comparing its host range, receptor usage and LTR functionality with other MLV isolates. We demonstrate using pseudotype experiments that XMRV Env mediates efficient infection of cells from different species. We show that replication competent XMRV infects various human cell types, including hematopoietic cell lines and prostate stromal fibroblasts. XMRV-LTR activity is significantly higher in the prostate cancer cell line LNCaP and in prostate stromal fibroblasts, compared to other cell types tested and could be one factor contributing to efficient viral spread in prostate tissue.
最近，首例人类感染外生性γ逆转录病毒（XMRV）的报道。在最初的描述中，XMRV 局限于前列腺基质成纤维细胞，尽管随后的报道表明 XMRV 蛋白在前列腺上皮细胞中表达。最近，XMRV 已在慢性疲劳综合征患者的血细胞中被检测到。本研究旨在通过比较 XMRV 与其他 MLV 分离株的宿主范围、受体使用和 LTR 功能，阐明 XMRV 的传播途径和组织嗜性。我们通过假型实验证明，XMRVEnv 介导不同物种细胞的有效感染。我们表明，复制型 XMRV 感染各种人类细胞类型，包括造血细胞系和前列腺基质成纤维细胞。与其他测试的细胞类型相比，XMRV-LTR 活性在前列腺癌细胞系 LNCaP 和前列腺基质成纤维细胞中显著更高，这可能是导致病毒在前列腺组织中有效传播的一个因素。
