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在血小板成熟过程中 GATA1 的缺失和 FLI1 的表达增加。

Loss of GATA1 and gain of FLI1 expression during thrombocyte maturation.

机构信息

Department of Biological Sciences, University of North Texas, Denton, 76203, USA.

出版信息

Blood Cells Mol Dis. 2010 Mar 15;44(3):175-80. doi: 10.1016/j.bcmd.2009.12.012. Epub 2010 Jan 27.

Abstract

In this paper, we characterized expression of GATA1 and FLI1 gene promoters in thrombocytes of zebrafish transgenic lines, G1-GM2 and TG(fli1:EGFP)y1 that carry transgenes of GATA1 and FLI1 gene promoters driving GFP. We found two discrete populations of thrombocytes verified by morphology, labeled with GFP in both G1-GM2 and TG(fli1:EGFP)y1 lines: (1) the more intensely labeled GFP+ thrombocyte, and (2) the less intensely labeled GFP+ thrombocytes. The more intensely labeled GFP+ thrombocyte in G1-GM2 line and the less intensely labeled GFP+ thrombocytes in the TG(fli1:EGFP)y1 line corresponded to young thrombocytes. These results showed that young thrombocytes have higher GATA1 promoter activity, while mature thrombocytes have more FLI1 gene promoter transcription. This finding suggests that there is a gradual loss of GATA1 and gain of FLI1 expression as the thrombocytes mature, and this overexpression of FLI1 may help maintain the thrombocyte lineage. Furthermore, the presence of transcriptional factors similar to those found in megakaryocytes raises the possibility that vertebrate thrombocytes may be the forerunners of mammalian megakaryocytes and, therefore, could serve as a model to study megakaryocyte maturation.

摘要

在本文中,我们对携带 GATA1 和 FLI1 基因启动子转基因的斑马鱼转基因系 G1-GM2 和 TG(fli1:EGFP)y1 的血小板中的 GATA1 和 FLI1 基因启动子表达进行了特征描述,这些转基因系驱动 GFP 的表达。我们发现了两种形态学验证的离散血小板群体,在 G1-GM2 和 TG(fli1:EGFP)y1 系中都用 GFP 标记:(1)更强烈标记的 GFP+血小板,和(2)较弱标记的 GFP+血小板。G1-GM2 系中更强烈标记的 GFP+血小板和 TG(fli1:EGFP)y1 系中较弱标记的 GFP+血小板对应于年轻的血小板。这些结果表明,年轻的血小板具有更高的 GATA1 启动子活性,而成熟的血小板具有更多的 FLI1 基因启动子转录。这一发现表明,随着血小板的成熟,GATA1 的表达逐渐减少,而 FLI1 的表达逐渐增加,这种 FLI1 的过表达可能有助于维持血小板谱系。此外,存在与巨核细胞中发现的转录因子相似的转录因子,这增加了脊椎动物血小板可能是哺乳动物巨核细胞的前身的可能性,因此可以作为研究巨核细胞成熟的模型。

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