Division of Microbiology, University of Bologna, Bologna, Italy.
Dermatology. 2010;220(2):138-42. doi: 10.1159/000277431. Epub 2010 Jan 26.
Parvovirus B19 is the aetiological agent of erythema infectiosum. The presence of B19 DNA in lesional skin of other cutaneous manifestations has frequently been reported although there is disagreement on the role of the B19 virus in tissues.
To investigate the presence of B19 DNA (1) in skin lesions of patients with a described B19-related disease, (2) in skin lesions of B19-unrelated diseases and (3) in healthy skin.
A total of 121 skin samples were examined for the presence of B19 DNA by PCR assays and peptide-nucleic-acid-based in situ hybridisation techniques.
B19 DNA was detected in 11/38 (28.9%) pityriasis lichenoides, 8/30 (26.7%) melanocytic naevi, 5/29 (17.2%) primary melanomas and 6/24 (25.0%) healthy skin biopsies. A difference in B19 DNA prevalence was observed in specimens grouped according to age, irrespective of pathologies.
B19 DNA can be found in skin tissues of patients with pityriasis lichenoides as well as in lesions not related to B19 infection and in healthy controls. B19 DNA can be detected in skin of young subjects in a significantly high rate compared to adults, suggesting that viral persistence may be the usual outcome after primary infection.
细小病毒 B19 是引起传染性红斑的病原体。虽然关于 B19 病毒在组织中的作用存在争议，但在其他皮肤表现的皮损中经常有 B19 DNA 的存在。
（1）检测在描述的与 B19 相关疾病的皮损中是否存在 B19 DNA；（2）检测在与 B19 无关的疾病的皮损中是否存在 B19 DNA；（3）检测在健康皮肤中是否存在 B19 DNA。
采用 PCR 检测和基于肽核酸的原位杂交技术，对 121 例皮肤样本进行 B19 DNA 检测。
在 38 例 pityriasis lichenoides （28.9%）、30 例黑色素痣 （26.7%）、29 例原发性黑素瘤 （17.2%）和 24 例健康皮肤活检样本 （25.0%）中检测到 B19 DNA。不论病理类型，根据年龄分组的标本中 B19 DNA 的流行率存在差异。
在 pityriasis lichenoides 患者的皮肤组织中，以及在与 B19 感染无关的皮损和健康对照中，都可以发现 B19 DNA。与成年人相比，年轻受试者的皮肤中 B19 DNA 的检出率明显较高，提示病毒持续存在可能是原发性感染后的常见结果。
Zotero 插件