• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

用带有紫外/可见分光光度检测器的流动注射法和用碘离子ISE 进行电势测定法在存在不同硫醇的情况下测定辅酶 A (CoASH)。

Determination of coenzyme A (CoASH) in the presence of different thiols by using flow-injection with a UV/Vis spectrophotometric detector and potentiometric determination of CoASH using an iodide ISE.

机构信息

Department of Analytical Chemistry, Faculty of Chemistry and Chemical Technology, Teslina 10/V, 21000 Split, Croatia.

出版信息

Molecules. 2009 Dec 29;15(1):100-13. doi: 10.3390/molecules15010100.

DOI:10.3390/molecules15010100
PMID:20110875
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6257127/
Abstract

Coenzyme A (CoA or CoASH) is one of the most important biologically active compounds, and for this reason a reliable, fast and simple determination of CoASH is needed. Here we are described a simple and fast way of determinating CoASH using potentiometric flow-injection analysis and spectrophotometric kinetic determination. The described methods are suitable for use over a wide concentration range (1 x 10(-6)-1 x 10(-4) M) of CoASH.

摘要

辅酶 A（CoA 或 CoASH）是最重要的生物活性化合物之一，因此需要一种可靠、快速和简单的 CoASH 测定方法。本文描述了一种使用电位流动注射分析和分光光度动力学测定法快速测定 CoASH 的简单方法。所描述的方法适用于 CoASH 宽浓度范围（1 x 10(-6)-1 x 10(-4) M）的测定。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6ba6/6257127/4d1ac665e992/molecules-15-00100-g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6ba6/6257127/e1245d882645/molecules-15-00100-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6ba6/6257127/b91c64b7cd3a/molecules-15-00100-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6ba6/6257127/d323c48dc5ca/molecules-15-00100-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6ba6/6257127/175956398b5a/molecules-15-00100-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6ba6/6257127/6dd08d3bc0b7/molecules-15-00100-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6ba6/6257127/1311426635b7/molecules-15-00100-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6ba6/6257127/36f919d3f719/molecules-15-00100-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6ba6/6257127/05fd12351c83/molecules-15-00100-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6ba6/6257127/b458c437ea2d/molecules-15-00100-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6ba6/6257127/4d1ac665e992/molecules-15-00100-g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6ba6/6257127/e1245d882645/molecules-15-00100-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6ba6/6257127/b91c64b7cd3a/molecules-15-00100-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6ba6/6257127/d323c48dc5ca/molecules-15-00100-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6ba6/6257127/175956398b5a/molecules-15-00100-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6ba6/6257127/6dd08d3bc0b7/molecules-15-00100-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6ba6/6257127/1311426635b7/molecules-15-00100-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6ba6/6257127/36f919d3f719/molecules-15-00100-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6ba6/6257127/05fd12351c83/molecules-15-00100-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6ba6/6257127/b458c437ea2d/molecules-15-00100-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6ba6/6257127/4d1ac665e992/molecules-15-00100-g010.jpg

相似文献

1
Determination of coenzyme A (CoASH) in the presence of different thiols by using flow-injection with a UV/Vis spectrophotometric detector and potentiometric determination of CoASH using an iodide ISE.用带有紫外/可见分光光度检测器的流动注射法和用碘离子ISE 进行电势测定法在存在不同硫醇的情况下测定辅酶 A (CoASH)。
Molecules. 2009 Dec 29;15(1):100-13. doi: 10.3390/molecules15010100.
2
Construction and performance characterization of ion-selective electrodes for potentiometric determination of pseudoephedrine hydrochloride applying batch and flow injection analysis techniques.应用分批和流动注射分析技术的电位法测定盐酸伪麻黄碱的离子选择性电极的构建及性能表征。
Ann Chim. 2006 Jul-Aug;96(7-8):421-33. doi: 10.1002/adic.200690043.
3
A highly sensitive PVC membrane iodide electrode based on complexes of mercury(II) as neutral carrier.一种基于汞（II）配合物作为中性载体的高灵敏度聚氯乙烯膜碘离子电极。
Anal Bioanal Chem. 2004 Sep;380(2):333-8. doi: 10.1007/s00216-004-2772-1. Epub 2004 Aug 5.
4
Sequential flow injection determination of chlorine species using a triiodide-selective electrode detector.使用三碘化物选择性电极检测器对氯物种进行顺序流动注射测定。
Anal Sci. 2006 Jan;22(1):45-50. doi: 10.2116/analsci.22.45.
5
Enzymatically modified ion-selective electrodes for flow injection analysis.用于流动注射分析的酶修饰离子选择电极。
J Pharm Biomed Anal. 1999 Mar;19(3-4):633-8. doi: 10.1016/s0731-7085(98)00230-1.
6
New conventional coated-wire ion-selective electrodes for flow-injection potentiometric determination of chlordiazepoxide.用于流动注射电位法测定氯氮卓的新型常规涂丝离子选择电极。
Anal Sci. 2005 Sep;21(9):1037-42. doi: 10.2116/analsci.21.1037.
7
Standard addition flow method for potentiometric measurements at low concentration levels: application to the determination of fluoride in food samples.低浓度水平电位测量的标准加入流动法：应用于食品样品中氟化物的测定
Talanta. 2015 Feb;133:1-6. doi: 10.1016/j.talanta.2013.12.058. Epub 2014 Jan 2.
8
Periodate oxidation analysis of carbohydrates. Part III. Potentiometric determination of periodate consumption by use of an iodide-selective electrode.
Anal Chim Acta. 1975 Jul;77:274-7. doi: 10.1016/S0003-2670(01)95180-3.
9
Cyanex based uranyl sensitive polymeric membrane electrodes.基于 Cynex 的铀敏聚合物膜电极。
Talanta. 2014 Jan;118:147-55. doi: 10.1016/j.talanta.2013.10.011. Epub 2013 Oct 19.
10
Propionyl-CoA condensing enzyme from Ascaris muscle mitochondria. II. Coenzyme A modulation.来自蛔虫肌肉线粒体的丙酰辅酶A缩合酶。II. 辅酶A调节。
Arch Biochem Biophys. 1991 Feb 15;285(1):166-71. doi: 10.1016/0003-9861(91)90345-j.

引用本文的文献

1
Differences in substrate specificity of V. cholerae FabH enzymes suggest new approaches for the development of novel antibiotics and biofuels.霍乱弧菌 FabH 酶的底物特异性差异为新型抗生素和生物燃料的开发提供了新的思路。
FEBS J. 2018 Aug;285(15):2900-2921. doi: 10.1111/febs.14588. Epub 2018 Jun 30.
2
Determination of Coenzyme A and Acetyl-Coenzyme A in Biological Samples Using HPLC with UV Detection.使用 HPLC 结合 UV 检测法测定生物样品中的辅酶 A 和乙酰辅酶 A。
Molecules. 2017 Aug 23;22(9):1388. doi: 10.3390/molecules22091388.
3
Functional screening and in vitro analysis reveal thioesterases with enhanced substrate specificity profiles that improve short-chain fatty acid production in Escherichia coli.

本文引用的文献

1
High-efficiency DNA separation by capillary electrophoresis in a polymer solution with ultralow viscosity.在超低粘度聚合物溶液中通过毛细管电泳实现高效 DNA 分离。
Anal Chem. 1999 Jul 1;71(13):2385-9. doi: 10.1021/ac990160x.
2
Spectrofluorimetric determination of trace amounts of coenzyme A using a terbium ion-ciprofloxacin complex probe in the presence of periodic acid.在高碘酸存在下，使用铽离子-环丙沙星络合物探针荧光分光光度法测定痕量辅酶A。
Anal Bioanal Chem. 2007 Mar;387(6):2083-9. doi: 10.1007/s00216-006-1075-0. Epub 2007 Jan 17.
3
Separation and quantitation of short-chain coenzyme A's in biological samples by capillary electrophoresis.
功能筛选和体外分析揭示了具有增强的底物特异性谱的硫酯酶，可提高大肠杆菌中短链脂肪酸的产量。
Appl Environ Microbiol. 2014 Feb;80(3):1042-50. doi: 10.1128/AEM.03303-13. Epub 2013 Nov 22.
通过毛细管电泳对生物样品中的短链辅酶A进行分离和定量分析。
Anal Chem. 2003 Jan 1;75(1):78-82. doi: 10.1021/ac0261505.
4
Application of a new method for the sensitive detection and quantification of acyl-CoA esters in Arabidopsis thaliana seedlings and mature leaves.一种用于灵敏检测和定量拟南芥幼苗及成熟叶片中酰基辅酶A酯的新方法的应用。
Biochem Soc Trans. 2000 Dec;28(6):575-7.
5
Technical Advance: a novel technique for the sensitive quantification of acyl CoA esters from plant tissues.技术进展：一种用于灵敏定量植物组织中酰基辅酶A酯的新技术。
Plant J. 2001 Jan;25(1):115-25. doi: 10.1046/j.1365-313x.2001.00929.x.
6
Pteridine analysis in urine by capillary electrophoresis using laser-induced fluorescence detection.采用激光诱导荧光检测的毛细管电泳法分析尿液中的蝶啶。
Anal Chem. 1999 Apr 1;71(7):1265-9. doi: 10.1021/ac981218v.
7
Determination of vitamin A in dried human blood spots by high-performance capillary electrophoresis with laser-excited fluorescence detection.采用激光激发荧光检测的高效毛细管电泳法测定干血斑中的维生素A
J Chromatogr B Biomed Appl. 1995 Mar 10;665(1):89-96. doi: 10.1016/0378-4347(94)00520-f.
8
Quantitative determination of serum iron in human blood by high-performance capillary electrophoresis.高效毛细管电泳法定量测定人血清铁
J Chromatogr B Biomed Appl. 1995 Jul 7;669(1):45-51. doi: 10.1016/0378-4347(95)00041-g.
9
A simple radioisotopic assay of acetylcarnitine and acetyl-CoA at picomolar levels.一种用于检测皮摩尔水平乙酰肉碱和乙酰辅酶A的简单放射性同位素测定法。
Anal Biochem. 1981 Mar 15;112(1):30-8. doi: 10.1016/0003-2697(81)90256-6.
10
Radioisotopic assay of picomolar amounts of coenzyme A.皮摩尔量辅酶A的放射性同位素测定法。
Anal Biochem. 1983 Oct 15;134(2):325-9. doi: 10.1016/0003-2697(83)90305-6.