Grum F, Paine D, Zoeller L
Appl Opt. 1972 Jan 1;11(1):93-8. doi: 10.1364/AO.11.000093.
Derivative spectrophotometry has been shown to have many important applications: for example, studying composition and reaction processes; providing gas signatures; detecting trace chemicals. This technique can become a powerful means for analyzing isomers used in polymer production. In this report, practical examples are given which typify applications of the derivative spectra. Conventional absorption and emission spectra often present overlapping bands not easily resolved by conventional means; band resolution usually is facilitated by first-and second-derivative spectra obtained from spectrophotometric measurements. Numerical methods based on both off-line and on-line computer processing are presented for generating first-and second-derivative spectra, and these techniques are discussed fully. With these methods, the contribution of background noise is emphasized. Ways to reduce this noise are given.
例如，研究成分和反应过程；提供气体特征；检测痕量化学物质。该技术可成为分析聚合物生产中所用异构体的有力手段。本报告给出了典型的导数光谱应用实例。传统的吸收光谱和发射光谱常常呈现出常规方法不易分辨的重叠谱带；通过分光光度测量获得的一阶和二阶导数光谱通常有助于谱带的分辨。介绍了基于离线和在线计算机处理生成一阶和二阶导数光谱的数值方法，并对这些技术进行了全面讨论。通过这些方法，突出了背景噪声的影响，并给出了降低这种噪声的方法。
