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涂层球体的光散射:生物细胞模型

Light scattering from coated spheres: model for biological cells.

作者信息

Brunsting A, Mullaney P F

出版信息

Appl Opt. 1972 Mar 1;11(3):675-80. doi: 10.1364/AO.11.000675.

DOI:10.1364/AO.11.000675
PMID:20111566
Abstract

Efficient methods for the calculation of light scatteringintensityfunctions for concentrically coated spheres (10-micro diam) are discussed. This model represents many types of biological cells whose nuclei have a low refractive index (1.1) and cytoplasms with a slightly lower refractive index. Studies are made on the relationships between the scattering coefficients for nonabsorbing, spherically symmetric scatterers. The physical origin of these coefficients is examined for absorbing scatterers. A comparison of the angular half-width of the scattered intensity functions for the coated sphere and an equivalent homogeneous sphere shows that diffraction dominates the small angle scattering in both cases. At larger angles, the coated sphere scattering pattern is more structured and quite sensitive to core sphere size, suggesting a possible method of distinguishing types of biological cells that are similar in gross size but different in internal detail.

摘要

讨论了计算同心涂层球体(直径约10微米)光散射强度函数的有效方法。该模型代表了许多类型的生物细胞,其细胞核具有低折射率(约1.1),细胞质的折射率略低。研究了非吸收性、球对称散射体的散射系数之间的关系。研究了吸收性散射体这些系数的物理来源。对涂层球体和等效均匀球体的散射强度函数的角半高宽进行比较表明,在两种情况下,衍射在小角度散射中占主导地位。在较大角度下,涂层球体的散射图案更具结构,并且对核心球体尺寸非常敏感,这表明可能存在一种区分总体尺寸相似但内部细节不同的生物细胞类型的方法。

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