Department of Microbiology and Molecular Genetics, University of the Punjab, Lahore, Pakistan.
Curr Microbiol. 2010 Aug;61(2):148-56. doi: 10.1007/s00284-010-9591-8. Epub 2010 Jan 29.
Dental unit water line (DUWL) contamination by opportunistic pathogens has its significance in nosocomial infection of patients, health care workers, and life-threatening infections to immunocompromized persons. Recently, the quorum sensing (QS) system of DUWL isolates has been found to affect their biofilm-forming ability, making it an attractive target for antimicrobial therapy. In this study, the effect of two quorum-sensing inhibitory compounds (patulin; PAT, penicillic acid; PA) and EDTA on planktonic growth, AI-2 signalling and in vitro biofilm formation of Pseudomonas aeruginosa, Achromobacter xylosoxidans and Achromobacter sp. was monitored. Vibrio harveyi BB170 bioassay and crystal violet staining methods were used to detect the AI-2 monitoring and biofilm formation in DUWL isolates, respectively. The V. harveyi BB170 bioassay failed to induce bioluminescence in A. xylosoxidans and Achromobacter sp., while P. aeruginosa showed AI-2 like activity suggesting the need of some pretreatments prior to bioassay. All strains were found to form biofilms within 72 h of incubation. The QSIs/EDTA combination have isolate-specific effects on biofilm formation and in some cases it stimulated biofilm formation as often as it was inhibited. However, detailed information about the anti-biofilm effect of these compounds is still lacking.
牙科治疗用水管路（DUWL）中机会性病原体的污染与患者、医护人员的医院感染以及免疫功能低下人群的生命威胁性感染有关。最近，发现 DUWL 分离株的群体感应（QS）系统会影响其生物膜形成能力，使其成为抗菌治疗的有吸引力的靶标。在这项研究中，监测了两种群体感应抑制化合物（棒曲霉素；PAT，青霉素酸；PA）和 EDTA 对铜绿假单胞菌、木糖氧化无色杆菌和无色杆菌属的浮游生物生长、AI-2 信号和体外生物膜形成的影响。使用 V. harveyi BB170 生物测定法和结晶紫染色法分别检测 DUWL 分离株中的 AI-2 监测和生物膜形成。V. harveyi BB170 生物测定法未能诱导木糖氧化无色杆菌和无色杆菌属生物发光，而铜绿假单胞菌则表现出 AI-2 样活性，表明在进行生物测定之前需要进行一些预处理。所有菌株在孵育 72 小时内均形成生物膜。QSIs/EDTA 组合对生物膜形成具有分离株特异性影响，在某些情况下，它会像抑制生物膜形成一样刺激生物膜形成。然而，这些化合物的抗生物膜作用的详细信息仍然缺乏。
