Key Laboratory of the Ministry of Education for Cell Biology and Tumor Cell Engineering, School of Life Science, Xiamen University, Xiamen, Fujian, People's Republic of China.
Parasitol Res. 2010 Mar;106(4):801-8. doi: 10.1007/s00436-010-1735-z. Epub 2010 Jan 29.
A full-length of complementary deoxyribonucleic acid (Tscdc42) encoding a putative Rho-family small GTPase gene cdc42 was isolated from Trichinella spiralis, an economically important parasitic nematode of zoonosis. The uninterrupted open reading frame of TsCDC42 encodes a predicted protein of 147 amino acids and containing a highly conserved domain of CDC42. Comparison with selected sequences from the free-living nematode Caenorhabditis elegans, Drosophila melanogaster, Xenopus laevis, Danio rerio, Mus musculus, and human showed that Tscdc42 is highly conserved. The highest identity of TsCDC42 with CDC42 from Drosophila is 67%, the similarity is up to 73%, the identity of TsCDC42 with the CDC42 homologue of C. elegans is 62%, and the similarity is up to 71%. Phylogenetic analysis of the amino acid sequence data, using the neighbor-joining and maximum parsimony methods, revealed that TsCDC42 is closely related to the molecule inferred from the cdc-42 gene of C. elegans. The transcript of TsCDC42 was analyzed during different stages of the worm.
从具有重要经济价值的动物寄生性线虫旋毛虫中分离出全长的互补脱氧核糖核酸（Tscdc42），该基因编码一个假定的Rho 家族小 GTP 酶基因 cdc42。TsCDC42 的连续开放阅读框编码一个预测的 147 个氨基酸的蛋白质，其中包含一个高度保守的 CDC42 结构域。与自由生活的线虫秀丽隐杆线虫、黑腹果蝇、非洲爪蟾、斑马鱼、小鼠和人类的选定序列进行比较表明，Tscdc42 高度保守。TsCDC42 与黑腹果蝇的 CDC42 同源性最高为 67%，相似性高达 73%，与秀丽隐杆线虫的 CDC42 同源物的同源性为 62%，相似性高达 71%。使用邻接法和最大简约法对氨基酸序列数据进行系统发育分析表明，TsCDC42 与从秀丽隐杆线虫的 cdc-42 基因推断出的分子密切相关。分析了 TsCDC42 在虫体不同发育阶段的转录情况。
