柠檬酸假单胞菌（3R）-羟基丁酸脱氢酶催化的氢向NAD转移的手性
Chirality of the Hydrogen transfer to NAD catalyzed by (3R) hydroxybutyrate dehydrogenase from Pseudomonas lemoignei.
作者信息
Alizade M A, Gaede K
出版信息
Z Naturforsch C Biosci. 1977 Sep-Oct;32(9-10):874-6. doi: 10.1515/znc-1977-9-1034.
The chirality of the hydrogen transfer from (3R) hydroxybutyrate to NAD catalyzed by (3R)hydroxybutyrate dehydrogenase (E.C. 1.1.1.30, D-3-hydroxybutyrate : NAD oxidoreductase) from Pseudomonas lemoignei was investigated. [4(-3)H] NAD was enzymatically reduced to (4R) [4(-3)H]NADH with (3RS) hydroxybutyrate. This observation was confirmed since NAD could be reduced to (4S) [4(-3)H] NADH with (3RS) [3(-3)H] hydroxybutyrate and (3R) hydroxybutyrate dehydrogenase. From these experiments it can be concluded that (3R) hydroxybutyrate dehydrogenase from P. lemoignei should be classified as an B or (S) type dehydrogenase.
研究了来自勒莫因假单胞菌的（3R）-羟基丁酸脱氢酶（E.C. 1.1.1.30，D-3-羟基丁酸：NAD氧化还原酶）催化的从（3R）-羟基丁酸到NAD的氢转移的手性。用（3RS）-羟基丁酸将[4(-3)H]NAD酶促还原为（4R）-[4(-3)H]NADH。这一观察结果得到了证实，因为用（3RS）-[3(-3)H]羟基丁酸和（3R）-羟基丁酸脱氢酶可将NAD还原为（4S）-[4(-3)H]NADH。从这些实验可以得出结论，来自勒莫因假单胞菌的（3R）-羟基丁酸脱氢酶应归类为B型或（S）型脱氢酶。
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