Faculty of Chemical and Food Technology SUT, Institute of Chemical and Environmental Engineering, Radlinského 9, 812 37, Bratislava, Slovakia.
Biotechnol Lett. 2010 May;32(5):675-80. doi: 10.1007/s10529-010-0203-2. Epub 2010 Jan 29.
Recombinant Escherichia coli cells, over-expressing cyclopentanone monooxygenase activity, were immobilized in polyelectrolyte complex capsules, made of sodium alginate, cellulose sulfate, poly(methylene-co-guanidine), CaCl(2) and NaCl. More than 90% of the cell viability was preserved during the encapsulation process. Moreover, the initial enzyme activity was fully maintained within encapsulated cells while it halved in free cells. Both encapsulated and free cells reached the end point of the Baeyer-Villiger biooxidation of 8-oxabicyclo[3.2.1]oct-6-en-3-one to 4,9-dioxabicyclo[4.2.1]non-7-en-3-one at the same time (48 h). Similarly, the enantiomeric excess above 94% was identical for encapsulated and free cells.
表达环戊酮单加氧酶活性的重组大肠杆菌细胞被固定在聚电解质复合胶囊中，该胶囊由海藻酸钠、纤维素硫酸酯、聚（亚甲撑-co-胍）、CaCl2和 NaCl 组成。在包封过程中，超过 90%的细胞活力得以保留。此外，在包封细胞中，初始酶活性得到了完全保持，而在游离细胞中则减半。包封细胞和游离细胞都在 8-氧杂双环[3.2.1]辛-6-烯-3-酮到 4,9-二氧杂双环[4.2.1]壬-7-烯-3-酮的 Baeyer-Villiger 生物氧化的终点同时达到（48 小时）。同样，包封细胞和游离细胞的对映体过量都超过了 94%。
