Department of Chemical Engineering, Polytechnic School, University of São Paulo, São Paulo, Brazil.
J Fluoresc. 2011 May;21(3):859-64. doi: 10.1007/s10895-009-0586-4. Epub 2010 Jan 29.
Crude petroleum oils are complex mixtures of different compounds (mainly organic), which are obtained from an extensive range of different geological sources. The fluorescence of crude petroleum oils derives largely from the aromatic hydrocarbon fraction, and this fluorescence emission is strongly influenced by the chemical composition (e.g., fluorophore and quencher concentrations) and physical characteristics (e.g., viscosity and optical density) of the oil. The fluorescence spectroscopy (FS) is increasingly used in petroleum technology due the availability of better optical detection techniques, because FS offers high sensitivity, good diagnostic potential, and relatively simple instrumentation. In this work we analyzed crude petroleum at different dilution in Nujol, a transparent mineral oil. The main objective of this work was to verify the possibility to measure crude oil emission spectroscopic without use of volatile solvents. The mixtures of nujol with different -crude oil concentrations were measured with a 10 mm optical path cuvette thus simplifying the fluorescence spectroscopy signal detection. The emission spectra were obtained by exciting the samples with a 400 W Xenon lamp at 350 nm, 450 nm and 532 nm. The emissions of the samples were collected perpendicularly with the excitation axis.
原油是由广泛的不同地质来源的不同化合物（主要是有机化合物）组成的复杂混合物。原油的荧光主要源于芳烃馏分，而这种荧光发射强烈地受到油的化学成分（例如，荧光团和猝灭剂浓度）和物理特性（例如，粘度和光密度）的影响。由于更好的光学检测技术的可用性，荧光光谱法（FS）在石油技术中得到了越来越多的应用，因为 FS 提供了高灵敏度、良好的诊断潜力和相对简单的仪器。在这项工作中，我们分析了不同稀释度的 Nujol 中的原油，Nujol 是一种透明的矿物油。这项工作的主要目的是验证在不使用挥发性溶剂的情况下测量原油发射光谱的可能性。用 10 毫米光程比色皿测量了 Nujol 与不同浓度原油的混合物，从而简化了荧光光谱信号的检测。通过在 350nm、450nm 和 532nm 处用 400W 氙灯激发样品来获得发射光谱。样品的发射光沿与激发轴垂直的方向收集。
