Department of Biological science, Sookmyung Women's University, Seoul, 140-742, Korea.
Mol Cells. 2010 Mar;29(3):239-44. doi: 10.1007/s10059-010-0022-2. Epub 2010 Jan 21.
The study of ES cell-mediated neuronal differentiation allows elucidating the mechanism of neuronal development in spite of the complexity and the difficult accessibility. During the differentiation of embryonic stem cells into neuronal cell, the expression profiles in the level of protein were extensively investigated by proteomic analysis. These cells were analyzed for charges in proteome during the differentiation of ES cells by 2-dimensional electrophoresis (2-DE) and MALDI-TOF MS. Seven unique proteins were identified, some of which were differentially expressed at each stage. A complex system of neuronal differentiation can be activated in cultured embryonic stem cells and our two dimensional electrophoresis data should be useful for investigating some of the mechanism that regulates neuronal differentiation.
胚胎干细胞介导的神经元分化研究可以阐明神经元发育的机制，尽管其过程复杂且难以研究。在胚胎干细胞向神经元细胞分化的过程中，通过蛋白质组学分析广泛研究了蛋白质水平的表达谱。通过二维电泳 (2-DE) 和 MALDI-TOF MS 分析这些细胞在 ES 细胞分化过程中的蛋白质组电荷。鉴定出 7 种独特的蛋白质，其中一些在每个阶段表达水平不同。在培养的胚胎干细胞中可以激活复杂的神经元分化系统，我们的二维电泳数据应该有助于研究一些调节神经元分化的机制。
