Department of Pharmacognosy, Research Institute for Pharmaceutical Sciences, School of Pharmacy, The University of Mississippi, University, Mississippi 38677, USA.
J Agric Food Chem. 2010 Feb 24;58(4):2180-7. doi: 10.1021/jf903762h.
Pomegranate juice derived ellagitannins and their intestinal bacterial metabolites, urolithins, inhibited TCDD-induced CYP1-mediated EROD activity in vitro with IC(50) values ranging from 56.7 microM for urolithin A to 74.8 microM for urolithin C. These compounds exhibited dose- and time-dependent decreases in cell proliferation and clonogenic efficiency of HT-29 cells. Inhibition of cell proliferation was mediated through cell cycle arrest in the G(0)/G(1) and G(2)/M stages of the cell cycle followed by induction of apoptosis. These results indicate that the ellagitannins and urolithins released in the colon upon consumption of pomegranate juice in considerable amounts could potentially curtail the risk of colon cancer development, by inhibiting cell proliferation and inducing apoptosis.
石榴汁中的鞣花单宁及其肠道细菌代谢产物尿石素抑制 TCDD 诱导的 CYP1 介导的 EROD 活性，IC50 值范围从 56.7μM 的尿石素 A 到 74.8μM 的尿石素 C。这些化合物表现出剂量和时间依赖性的 HT-29 细胞增殖和集落形成效率降低。细胞增殖的抑制是通过细胞周期阻滞在 G0/G1 和 G2/M 期，随后诱导细胞凋亡来介导的。这些结果表明，大量摄入石榴汁后在结肠中释放的鞣花单宁和尿石素可能通过抑制细胞增殖和诱导细胞凋亡来降低结肠癌发展的风险。
