Food Safety Programme, Christchurch Science Centre, Institute of Environmental Science and Research Limited, Ilam, Christchurch, New Zealand.
Foodborne Pathog Dis. 2010 Jun;7(6):687-93. doi: 10.1089/fpd.2009.0453.
The use of replication-deficient UV-treated bacteriophages, or phages, presents an alternative to viable phages for food biocontrol applications. Nontransducing UV-treated phages, if used correctly, are unlikely to produce viable progeny phages, which might otherwise mediate undesirable horizontal gene transfer events. Phage T4 and Escherichia coli were used as a model system to examine this possibility. UV-treated phages were able to cause a reduction in the optical density of outer membrane-free cell suspensions and they also killed host cells under conditions not permitting their multiplication, that is, 24 degrees C for 2 h and 37 degrees C for 15 min. Host cell reductions were also demonstrated in broth and on meat at 5 degrees C when high concentrations of phages of 2.3 x 10(9) PFU mL(-1) and 1.8 x 10(8) PFU cm(-2), respectively, were used. At 24 degrees C and 37 degrees C, "lysis from without" was likely to be the mechanism responsible for the reduction in host cell concentrations, but at 5 degrees C this may not have been the case.
使用复制缺陷的紫外线处理噬菌体（或噬菌体）作为一种替代有活力的噬菌体的方法，用于食品生物防治应用。如果使用得当，非转导紫外线处理的噬菌体不太可能产生有活力的子代噬菌体，否则可能介导不期望的水平基因转移事件。噬菌体 T4 和大肠杆菌被用作模型系统来检验这种可能性。紫外线处理的噬菌体能够导致去外膜细胞悬浮液的光密度降低，并且在不允许其繁殖的条件下也能杀死宿主细胞，即在 24°C 下 2 小时和 37°C 下 15 分钟。当使用浓度分别为 2.3 x 10(9)PFU mL(-1)和 1.8 x 10(8)PFU cm(-2)的噬菌体时，在 5°C 时也在肉汤中和肉上证明了宿主细胞减少。在 24°C 和 37°C 下，“从外到内的裂解”可能是导致宿主细胞浓度降低的原因，但在 5°C 下情况可能并非如此。
