Department of Plant Pathology, China Agricultural University, Beijing 100193, China.
J Eukaryot Microbiol. 2010 Mar-Apr;57(2):206-12. doi: 10.1111/j.1550-7408.2009.00467.x. Epub 2010 Jan 22.
The genetic variation among 128 isolates of Monilinia fructicola (Fungi, Ascomycota, Helotiales) from China was analyzed using Inter-Simple Sequence Repeat (ISSR) markers and compared with those of samples from California, USA and New Zealand. A total of 72 reproducible DNA fragments were scored, of which 87.5% (63/72) were polymorphic. The Nei's gene diversity and Shannon's diversity indices of three Chinese regional populations were very similar to that from California. However, several differences were observed among geographic populations of M. fructicola from both within China and between China and California. The analysis of molecular variance (AMOVA) of isolates from different geographic locations suggested that most of the observed genetic variation was found within populations. Results of this study are inconsistent with the hypothesis that the Chinese populations of M. fructicola were derived from a single or few recent migrants from other countries. Instead, our results suggest that M. fructicola has been in China long before its first official recording in 2003.
对来自中国的 128 株棒孢霉（真菌，子囊菌门，Helotiales）的遗传变异进行了分析，使用了简单重复序列间（ISSR）标记，并与来自美国加利福尼亚州和新西兰的样本进行了比较。共记录了 72 个可重复的 DNA 片段，其中 87.5%（63/72）具有多态性。三个中国地区种群的 Nei 基因多样性和 Shannon 多样性指数与加利福尼亚州的非常相似。然而，在中国内部和中国与加利福尼亚州之间的棒孢霉地理种群之间观察到了几个差异。对不同地理位置的分离株的分子方差分析（AMOVA）表明，观察到的遗传变异大部分存在于种群内部。本研究的结果与棒孢霉中国种群是由来自其他国家的单一或少数近期移民衍生而来的假设不一致。相反，我们的结果表明，棒孢霉在中国的存在时间远远早于 2003 年首次正式记录的时间。
