Zhang Ruiying, Huang Chenyang, Zheng Suyue, Zhang Jinxia, Ng Tzi Bun, Jiang Ruibo, Zuo Xuemei, Wang Hexiang
Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, Beijing 100081, China.
Appl Microbiol Biotechnol. 2007 Feb;74(1):140-5. doi: 10.1007/s00253-006-0628-7. Epub 2006 Dec 22.
To validate strain typing by inter simple sequence repeat (ISSR) analysis in Lentinula edodes cultivars, 17 Chinese L. edodes strains including 15 cultivated strains cultivated on a large scale and two wild strains were analyzed with the ISSR technique. With the use of two ISSR primers, a total of 32 DNA products were detected, of which, 31 DNA products (96.9% of the detected products) were polymorphic between two or more strains. The profiles of those two primers could be employed to differentiate all of the tested strains. A cluster analysis based on ISSR data revealed that the 17 strains could be classified into two distinct groups. One group consisted of eight strains in which the cultivated strains were H (high-temperature)-type or B (broad-temperature)-type, and the other group comprised cultivated strains that were of the L (low-temperature)-type or M (medium-temperature)-type. In contrast to the two wild strains, the genetic diversity of 15 cultivated strains was very rich based on a similarity coefficient analysis.
为了通过香菇品种的简单序列重复区间（ISSR）分析来验证菌株分型，我们使用ISSR技术对17个中国香菇菌株进行了分析，其中包括15个大规模栽培菌株和2个野生菌株。使用两个ISSR引物，共检测到32个DNA产物，其中31个DNA产物（占检测产物的96.9%）在两个或更多菌株之间具有多态性。这两个引物的图谱可用于区分所有测试菌株。基于ISSR数据的聚类分析表明，17个菌株可分为两个不同的组。一组由8个菌株组成，其中栽培菌株为H（高温）型或B（广温）型，另一组包括L（低温）型或M（中温）型的栽培菌株。与两个野生菌株相比，基于相似系数分析，15个栽培菌株的遗传多样性非常丰富。
