[T-bet和GATA3在儿童急性特发性血小板减少性紫癜中的表达]
[Expression of T-bet and GATA3 in children with acute idiopathic thrombocytopenic purpura].
作者信息
Ren Shao-Min, Zhang Chun-Xia, Li Wei-Cai
机构信息
Department of Peditrics, Affiliated Hospital of Innner Mongolia Medical College, Hohhot 010050, China.
出版信息
Zhongguo Dang Dai Er Ke Za Zhi. 2010 Jan;12(1):29-31.
OBJECTIVE
To study the expression of transcriptional factors T-bet and GATA3 mRNA and the levels of IFN-gamma and IL-4 in blood in children with acute idiopathic thrombocytopenic purpura (ITP) and investigate the tendency of polarization of Th1/Th2 in children with ITP.
METHODS
Blood T-bet and GATA3 mRNA expression were examined using RT-PCR and plasma IFN-gamma and IL-4 levels were measured using EIASA in children with acute ITP in acute (n=30) and remission stages (n=28). Twenty healthy children served as the controls.
RESULTS
Blood T-bet mRNA expression and IFN-gamma levels in children with ITP in the acute stage were markedly higher than those in the remission stage and controls (p<0.01). In contrast, blood GATA3 mRNA expression and IL-4 levels in children with ITP in the acute stage were significantly lower than those in the remmission stage and controls (p<0.01).
CONCLUSIONS
The high expression of T-bet and IFN-gamma and the low expression of GATA3 and IL-4 indicate the existence of Th1 polarization in children with acute ITP. This might be related to the regulation of T-bet and GATA3.
目的
研究急性特发性血小板减少性紫癜（ITP）患儿血液中转录因子T-bet和GATA3 mRNA的表达以及IFN-γ和IL-4水平，探讨ITP患儿Th1/Th2极化倾向。
方法
采用RT-PCR检测急性ITP患儿急性期（n = 30）和缓解期（n = 28）血液中T-bet和GATA3 mRNA的表达，采用酶联免疫吸附测定法（EIASA）检测血浆中IFN-γ和IL-4水平。选取20名健康儿童作为对照。
结果
急性ITP患儿急性期血液中T-bet mRNA表达和IFN-γ水平显著高于缓解期和对照组（p<0.01）。相反，急性ITP患儿急性期血液中GATA3 mRNA表达和IL-4水平显著低于缓解期和对照组（p<0.01）。
结论
T-bet和IFN-γ的高表达以及GATA3和IL-4的低表达表明急性ITP患儿存在Th1极化。这可能与T-bet和GATA3的调节有关。
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