OME患者积液中GATA3、T-bet、IL-4和IFN-γ mRNA的表达。
Expression of GATA3, T-bet, IL-4, and IFN-gamma mRNA in the effusion of OME patients.
作者信息
Shim Hyun Joon, Park Dong Choon, Lee Young Chan, Eun Young Gyu, Yeo Seung Geun
机构信息
Department of Otorhinolaryngology, School of Medicine, EulJi University, Seoul, Republic of Korea.
出版信息
Int J Pediatr Otorhinolaryngol. 2009 Aug;73(8):1119-23. doi: 10.1016/j.ijporl.2009.04.024. Epub 2009 May 29.
OBJECTIVE
The role of allergy in the pathophysiology of otitis media with effusion (OME) remains unclear. We evaluated the role of allergy and the Th1/Th2 balance in OME patients.
METHOD
Middle ear fluid was collected from 46 OME patients who had a ventilating tube inserted, and expression of GATA3, T-bet, IL-4, and IFN-gamma mRNA was assessed by RT-PCR. Expression of transcription factors and cytokines was compared in patients with and without allergy.
RESULTS
The levels of GATA3 and T-bet mRNA in effusion fluid correlated positively with the levels of IL-4 and IFN-gamma mRNA, respectively. However, the allergy and non-allergy groups showed no significant differences in expression of any of these transcription factors and cytokines. The T-bet:GATA3 ratio was higher in the non-allergy than in the allergy group, but the difference was not statistically significant.
CONCLUSION
Although we observed a significant positive correlation between GATA3 and IL-4 mRNA levels, we cannot conclude that OME with allergy is related to a Th-2 driven immune response.
目的
变应性在分泌性中耳炎（OME）病理生理学中的作用仍不明确。我们评估了变应性及Th1/Th2平衡在OME患者中的作用。
方法
从46例插入通气管的OME患者中收集中耳积液，采用逆转录聚合酶链反应（RT-PCR）评估GATA3、T-bet、白细胞介素-4（IL-4）和γ干扰素（IFN-γ）mRNA的表达。比较有和无变应性患者中转录因子和细胞因子的表达。
结果
积液中GATA3和T-bet mRNA水平分别与IL-4和IFN-γ mRNA水平呈正相关。然而，变应性组和非变应性组在这些转录因子和细胞因子的表达上均无显著差异。非变应性组的T-bet:GATA3比值高于变应性组，但差异无统计学意义。
结论
虽然我们观察到GATA3和IL-4 mRNA水平之间存在显著正相关，但我们不能得出变应性OME与Th2驱动的免疫反应相关的结论。
Zotero 插件