Wang Tuan-Mei, Bo Tao, Wang Man-Zhi, Zhu Xiao-Hua, Li Jian, Li Xing-Fang, Mao Ding-An
Department of Pediatrics, Central South University, Changsha 410011, China.
Zhongguo Dang Dai Er Ke Za Zhi. 2010 Jan;12(1):47-50.
To investigate the effets of flurothyl-induced neonatal recurrent seizures on glucocorticoid receptor (GR) expression in the rat brain.
Forty-eight seven-day-old Sprague-Dawley rats were randomly divided into two groups: control and seizure. Seizures were induced by inhalant flurothyl daily for six consecutive days. Brains were sampled on postnatal days 13, 15 and 19. The expression of GR protein in the cerebral cortex was detected by Western blot and immunohistochemical method.
The expression of GR in the cerebral cortical plasma protein was significantly lower in the seizure group than in the control group on postnatal day 15. The expression of GR protein in the cerebral cortical nuclear protein decreased significantly in the seizure group compared with that in the control group on postnatal days 15 and 19 (p<0.05). Compared to the control group, the accumulated optical density (AOD) of GR immunoreactivity (IR) decreased significantly in the parietal cortex on postnatal day 13 (p<0.05), the AOD of GR IR decreased significantly in the parietal cortex and the temporal cortex on postnatal day 15 (p<0.05), and the AOD of GR IR decreased significantly in the parietal cortex, temporal cortex and the frontal cortex in the seizure group on postnatal day 19 (p<0.05).
Recurrent seizures in neonatal rats result in abnormal GR expression in the cerebral cortex which might play an important role in short-term brain injury induced by early recurrent seizures.
探讨氟乙酰胺诱导的新生大鼠反复惊厥对大鼠脑内糖皮质激素受体（GR）表达的影响。
将48只7日龄的Sprague-Dawley大鼠随机分为两组：对照组和惊厥组。连续6天每日通过吸入氟乙酰胺诱导惊厥。在出生后第13、15和19天采集大脑样本。采用蛋白质免疫印迹法和免疫组织化学方法检测大脑皮质中GR蛋白的表达。
出生后第15天，惊厥组大脑皮质血浆蛋白中GR的表达显著低于对照组。出生后第15天和19天，惊厥组大脑皮质核蛋白中GR蛋白的表达与对照组相比显著降低（p<0.05）。与对照组相比，出生后第13天顶叶皮质中GR免疫反应性（IR）的累积光密度（AOD）显著降低（p<0.05），出生后第15天顶叶皮质和颞叶皮质中GR IR的AOD显著降低（p<0.05），出生后第19天惊厥组顶叶皮质、颞叶皮质和额叶皮质中GR IR的AOD显著降低（p<0.05）。
新生大鼠反复惊厥导致大脑皮质GR表达异常，这可能在早期反复惊厥所致的短期脑损伤中起重要作用。
