Rosenberg C L, Kim H G, Shows T B, Kronenberg H M, Arnold A
Endocrine Unit, Massachusetts General Hospital, Boston 02114.
Oncogene. 1991 Mar;6(3):449-53.
We report the detailed molecular characterization of a human parathyroid adenoma with a clonal parathyroid hormone gene rearrangement. This rearrangement is similar to one we characterized recently in an independent adenoma. In these two, plus a third partially characterized adenoma, one allele of the PTH gene, on 11p15, is rearranged with DNA from the D11S287 region on 11q13. This region contains a transcribed sequence, D11S287E, distinct from known 11q13 oncogenes, that is expressed in all parathyroid tissues examined, but is overexpressed dramatically in all three tumours with PTH gene-D11S287 rearrangements. These findings suggest that overexpression of D11S287E, perhaps driven by the misplaced PTH gene's regulatory elements, contributed to the development of these benign tumors. D11S287E is a new candidate oncogene with potential importance in parathyroid adenomas and perhaps other tumors with 11q13 abnormalities.
我们报告了一例具有克隆性甲状旁腺激素基因重排的人甲状旁腺腺瘤的详细分子特征。这种重排类似于我们最近在另一例独立腺瘤中所鉴定的重排。在这两例以及第三例部分特征已明确的腺瘤中，位于11p15的甲状旁腺激素（PTH）基因的一个等位基因与位于11q13的D11S287区域的DNA发生了重排。该区域包含一个转录序列D11S287E，它不同于已知的11q13致癌基因，在所检测的所有甲状旁腺组织中均有表达，但在所有三例发生PTH基因 - D11S287重排的肿瘤中显著过表达。这些发现表明，可能由错位的PTH基因调控元件驱动的D11S287E过表达，促成了这些良性肿瘤的发生。D11S287E是一个新的候选致癌基因，在甲状旁腺腺瘤以及可能其他具有11q13异常的肿瘤中具有潜在重要性。
