中性粒细胞在鼠疫耶尔森菌引起的肺部感染的早期控制中起重要作用。

Neutrophils are important in early control of lung infection by Yersinia pestis.

机构信息

Defence Science and Technology Laboratory, Porton Down, Salisbury, Wiltshire, UK.

出版信息

Microbes Infect. 2010 Apr;12(4):331-5. doi: 10.1016/j.micinf.2010.01.007. Epub 2010 Jan 28.

DOI:10.1016/j.micinf.2010.01.007

PMID:20114086

Abstract

In this paper we evaluate the role of neutrophils in pneumonic plague. Splenic neutrophils from naïve BALB/c mice were found to reduce numbers of culturable Yersinia pestis strain GB in suspension. A murine, BALB/c, intranasal model of pneumonic plague was used in conjunction with in vivo neutrophil ablation, using the GR-1 antibody. This treatment reduced neutrophil numbers without affecting other leukocyte numbers. Neutrophil ablated mice exhibited increased bacterial colonisation of the lung 24h post infection. Furthermore, exposure of Y. pestis to human neutrophils resulted in a 5-fold reduction in the number of viable bacterial cells, whereas, PBMCs had no effect.

摘要

在本文中，我们评估了中性粒细胞在肺鼠疫中的作用。从天真的 BALB/c 小鼠的脾脏中性粒细胞中发现，它们减少了悬浮液中可培养的鼠疫耶尔森菌 GB 的数量。使用 GR-1 抗体，结合体内中性粒细胞耗竭，建立了 BALB/c 小鼠的鼻内肺炎型鼠疫模型。这种治疗方法减少了中性粒细胞数量，而不影响其他白细胞数量。中性粒细胞耗竭的小鼠在感染后 24 小时表现出肺部细菌定植增加。此外，人中性粒细胞暴露于鼠疫耶尔森菌导致活菌细胞数量减少了 5 倍，而 PBMC 则没有影响。