An improved bacterial colony lysis procedure enables direct DNA hybridisation using short (10, 11 bases) oligonucleotides to cosmids.

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作者信息

Nizetic D, Drmanac R, Lehrach H

机构信息

Genome Analysis Laboratory, Imperial Cancer Research Fund, London, UK.

出版信息

Nucleic Acids Res. 1991 Jan 11;19(1):182. doi: 10.1093/nar/19.1.182.

DOI:10.1093/nar/19.1.182

PMID:2011501

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC333552/

Abstract

摘要

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b1d5/333552/d62cdcc7019b/nar00237-0186-a.jpg

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本文引用的文献

Detection of genes for heat-stable enterotoxin I in Escherichia coli strains isolated in Brazil.巴西分离的大肠杆菌菌株中热稳定肠毒素I基因的检测。

Infect Immun. 1985 Jul;49(1):46-51. doi: 10.1128/iai.49.1.46-51.1985.

Molecular approaches to genome analysis: a strategy for the construction of ordered overlapping clone libraries.基因组分析的分子方法：构建有序重叠克隆文库的策略。

Comput Appl Biosci. 1987 Sep;3(3):203-10. doi: 10.1093/bioinformatics/3.3.203.

The complete DNA sequence of the long unique region in the genome of herpes simplex virus type 1.单纯疱疹病毒1型基因组中长单一区域的完整DNA序列。

J Gen Virol. 1988 Jul;69 ( Pt 7):1531-74. doi: 10.1099/0022-1317-69-7-1531.

Reliable hybridization of oligonucleotides as short as six nucleotides.短至六个核苷酸的寡核苷酸的可靠杂交。

DNA Cell Biol. 1990 Sep;9(7):527-34. doi: 10.1089/dna.1990.9.527.

Nucleic Acids Res. 1990 May 11;18(9):2653-60. doi: 10.1093/nar/18.9.2653.

Colony hybridization: a method for the isolation of cloned DNAs that contain a specific gene.菌落杂交：一种分离含有特定基因的克隆DNA的方法。

Proc Natl Acad Sci U S A. 1975 Oct;72(10):3961-5. doi: 10.1073/pnas.72.10.3961.

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