Department of Otolaryngology-Head and Neck Surgery, University of North Carolina School of Medicine, Chapel Hill, NC 27599-7070, USA.
Otolaryngol Head Neck Surg. 2010 Feb;142(2):260-5. doi: 10.1016/j.otohns.2009.10.006.
To investigate the ability to detect the in vivo cochlear changes associated with vasopressin-induced and surgically induced endolymphatic hydrops using MRI at 3 tesla (T).
Prospective, animal model.
Animal laboratory.
In group 1, five guinea pigs underwent post-gadolinium temporal bone MRI before and after seven and 14 days of chronic systemic administration of vasopressin by osmotic pump. In group 2, five guinea pigs underwent temporal bone MRI eight weeks after unilateral surgical ablation of the endolymphatic sac. Three-tesla high-resolution T1-weighted sequences were acquired pre- and postcontrast administration. Region of interest signal intensities of the perilymph and endolymph were analyzed manually. Quantitative evaluation of hydrops was measured histologically.
Gadolinium preferentially concentrated in the perilymph, allowing for distinction of cochlear compartments on 3.0-T MRI. The T1-weighted contrast MRI of vasopressin-induced hydropic cochlea showed significant increases in signal intensity of the endolymph and perilymph. Surgically induced unilateral hydropic cochlea also showed increased signal intensity, compared with the control cochlea of the same animal, but less of an increase than the vasopressin group. The histological degree of hydrops induced in the vasopressin group was comparable to previous studies.
In vivo postcontrast MRI of the inner ear demonstrated cochlear changes associated with chronic systemic administration of vasopressin and surgical ablation of the endolymphatic sac. Understanding the MRI appearance of endolymphatic hydrops induced by various methods contributes to the future use of MRI as a possible tool in the diagnosis and treatment of Ménière's disease.
在 3 特斯拉（T）下使用 MRI 研究加压素诱导和手术引起的内淋巴积水的体内耳蜗变化的检测能力。
前瞻性动物模型。
动物实验室。
在第 1 组中，5 只豚鼠在慢性全身给予加压素通过渗透泵治疗前后 7 天和 14 天进行颞骨 MRI 检查。在第 2 组中，5 只豚鼠在单侧内淋巴囊手术消融 8 周后进行颞骨 MRI 检查。在给药前和给药后采集 3T 高分辨率 T1 加权序列。手动分析外淋巴和内淋巴的感兴趣区信号强度。通过组织学定量评估积水。
钆优先积聚在外淋巴中，允许在 3.0-T MRI 上区分耳蜗室。加压素诱导的积水耳蜗的 T1 加权对比 MRI 显示内淋巴和外淋巴的信号强度显著增加。与同一动物的对照耳蜗相比，手术引起的单侧积水耳蜗也显示出信号强度增加，但增加幅度小于加压素组。加压素组诱导的积水程度与先前的研究相当。
内耳的体内对比 MRI 显示了与慢性全身给予加压素和手术消融内淋巴囊相关的耳蜗变化。了解各种方法引起的内淋巴积水的 MRI 表现有助于将来将 MRI 用作梅尼埃病诊断和治疗的一种可能工具。
