随着人类胶质瘤肿瘤分级的升高，Akt2的表达增加、PI3K的活性增强以及细胞增殖加快。
Increased expression of Akt2 and activity of PI3K and cell proliferation with the ascending of tumor grade of human gliomas.
作者信息
Wang Guangxiu, Kang Chunsheng, Pu Peiyu
机构信息
Department of Neurosurgery, Tianjin Medical University General Hospital, Laboratory of Neuro-oncology, Tianjin Neurological Institute, 154# An Shan Road, Tianjin, 300052, People's Republic of China.
出版信息
Clin Neurol Neurosurg. 2010 May;112(4):324-7. doi: 10.1016/j.clineuro.2010.01.003. Epub 2010 Feb 8.
OBJECTIVE
To study the expression of Akt2 and activation of PI3K in different grades of human gliomas and correlate the Akt2 expression with the proliferation activity of gliomas.
METHODS
Akt2 expression and PI3K activity were examined in 48 different grades of human glioma specimens and six normal brain tissue samples by immunohistochemistry and Western blot analysis. The proliferation activity of tumors was evaluated by Ki-67 nuclear antigen labeling index (Ki-67 LI) using immunostaining.
RESULTS
In contrast to the normal brain tissues, Akt2 expression and PI3K activity were greatly increased with the ascending of tumor grade and correlated positively with the proliferation activity of gliomas.
CONCLUSION
Akt2 may play a critical role in the development of gliomas and present a potential therapeutic target for malignant gliomas.
目的
研究Akt2在不同级别人类胶质瘤中的表达以及PI3K的激活情况，并将Akt2表达与胶质瘤的增殖活性相关联。
方法
采用免疫组织化学和蛋白质印迹分析检测48例不同级别人类胶质瘤标本及6例正常脑组织样本中的Akt2表达和PI3K活性。通过免疫染色用Ki-67核抗原标记指数（Ki-67 LI）评估肿瘤的增殖活性。
结果
与正常脑组织相比，Akt2表达和PI3K活性随肿瘤级别的升高而显著增加，且与胶质瘤的增殖活性呈正相关。
结论
Akt2可能在胶质瘤的发生发展中起关键作用，是恶性胶质瘤潜在的治疗靶点。
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