Gatignol A, Buckler-White A, Berkhout B, Jeang K T
Laboratory of Molecular Microbiology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892.
Science. 1991 Mar 29;251(5001):1597-600. doi: 10.1126/science.2011739.
Human immunodeficiency virus type 1 (HIV-1) gene expression is activated by Tat, a virally encoded protein. Tat trans-activation requires viral (trans-activation--responsive; TAR) RNA sequences located in the R region of the long terminal repeat (LTR). Existing evidence suggests that Tat probably cooperates with cellular factors that bind to TAR RNA in the overall trans-activation process. A HeLa complementary DNA was isolated and characterized that encodes a TAR RNA-binding protein (TRBP). TRBP activated the HIV-1 LTR and was synergistic with Tat function.
1型人类免疫缺陷病毒（HIV-1）的基因表达由病毒编码的蛋白质Tat激活。Tat反式激活需要位于长末端重复序列（LTR）R区域的病毒（反式激活应答元件；TAR）RNA序列。现有证据表明，在整个反式激活过程中，Tat可能与结合TAR RNA的细胞因子协同作用。分离并鉴定了一种编码TAR RNA结合蛋白（TRBP）的HeLa互补DNA。TRBP激活HIV-1 LTR，并与Tat功能具有协同作用。
