Department of Pharmacy, Shinshu University Hospital, 3-1-1 Asahi, Matsumoto 390-8621, Japan.
Biol Pharm Bull. 2010;33(2):249-54. doi: 10.1248/bpb.33.249.
Expression levels of cytochrome P450 (CYP) 3A4, CYP3A5 and CYP3A7 mRNAs in placentas and fetal membranes, which were split into amnion and chorion leave attached decidua (chorion/decidua), obtained from pregnant women with normal delivery (5 subjects) and Caesarean section (15 subjects) were determined. These CYP3A mRNAs were also expressed in amnion and chorion/decidua together with placenta, although the expression level of these mRNAs was strikingly different between subjects. The expression level of the CYP3A4 mRNA in the placenta was about 2-fold higher than those in amnion and chorion/decidua. On the other hand, the expression levels of CYP3A5 and CYP3A7 mRNAs were highest in chorion/decidua. The immunologically related protein(s) with CYP3A7 was detected in all tissues examined. Testosterone 6beta-hydroxylase activity in homogenate of human placenta, amnion and chorion/decidua were 26.6, 3.7 and 4.6 pmol/h/mg protein, respectively. These results suggest that CYP3As in fetal membranes have the metabolic function to protect the fetus from exposure to drugs.
从行剖宫产术（15 例）和正常分娩（5 例）的孕妇的胎盘、胎膜（分为羊膜和绒毛膜连胎盘组织）中提取细胞色素 P450（CYP）3A4、CYP3A5 和 CYP3A7mRNA，并对其进行检测。这些 CYP3A 信使 RNA 与胎盘、羊膜和绒毛膜连胎盘组织一起表达，尽管这些信使 RNA 在不同受试者中的表达水平有明显差异。胎盘 CYP3A4mRNA 的表达水平约为羊膜和绒毛膜连胎盘组织的 2 倍。另一方面，CYP3A5 和 CYP3A7mRNA 的表达水平在绒毛膜连胎盘组织中最高。在所有检测的组织中均检测到与 CYP3A7 免疫相关的蛋白质。人胎盘、羊膜和绒毛膜连胎盘组织匀浆中的睾酮 6β-羟化酶活性分别为 26.6、3.7 和 4.6pmol/h/mg 蛋白。这些结果表明，胎膜中的 CYP3A 具有代谢功能，可以保护胎儿免受药物暴露。
