Gong Wei, Si Ke, Sheppard Colin J R
Division of Bioengineering, National University of Singapore, Singapore 117574.
Appl Opt. 2010 Feb 1;49(4):752-7. doi: 10.1364/AO.49.000752.
We present a diffraction analysis for image formation in fluorescence confocal microscopy with divided apertures. The three-dimensional optical transform function is given, and axial resolution and transverse resolution are investigated. The results show that the employment of a divided-aperture technique in fluorescence confocal microscopy can enhance the rejection of background scattering. In addition, the axial resolution and transverse resolution can be improved by adjusting the width of the divider strip. For a given detector size, an optimum value of the divider strip width is given to obtain the best axial resolution or transverse resolution. The integrated intensity and signal-to-background ratio are also discussed.
我们给出了用于具有分割孔径的荧光共聚焦显微镜成像的衍射分析。给出了三维光学传递函数，并研究了轴向分辨率和横向分辨率。结果表明，在荧光共聚焦显微镜中采用分割孔径技术可以增强对背景散射的抑制。此外，通过调整分割条的宽度可以提高轴向分辨率和横向分辨率。对于给定的探测器尺寸，给出了分割条宽度的最佳值以获得最佳的轴向分辨率或横向分辨率。还讨论了积分强度和信噪比。
