Institute of Medical Microbiology and Hygiene, TU Dresden, Dresden, Germany.
Eur J Clin Microbiol Infect Dis. 2010 Apr;29(4):481-7. doi: 10.1007/s10096-009-0872-8. Epub 2010 Jan 30.
A total of 57 isolates of Legionella pneumophila were randomly selected from the German National Legionella strain collection and typed by monoclonal antibody subgrouping, seven-gene locus sequence-based typing (SBT) scheme and a newly developed variable element typing (VET) system based on the presence or absence of ten variable genetic elements. These elements were detected while screening a genomic library of strain Corby, as well as being taken from published data for PAI-1 (pathogenicity island) from strain Philadelphia. Specific primers were designed and used in gel-based polymerase chain reaction (PCR) assays. PCR amplification of the mip gene served as a control. The end-point was the presence/absence of a PCR product on an ethidium bromide-strained gel. In the present study, the index of discrimination was somewhat lower than that of the SBT (0.87 versus 0.97). Nevertheless, the results obtained showed as a 'proof of principle' that this simple and quick typing assay might be useful for the epidemiological characterisation of L. pneumophila strains.
从德国国家军团菌株收藏中随机选择了 57 株嗜肺军团菌分离株，并通过单克隆抗体亚群分型、7 基因座序列分型（SBT）方案和新开发的基于十个可变遗传元件存在或缺失的可变元件分型（VET）系统进行分型。这些元件是在筛选菌株 Corby 的基因组文库时检测到的，并且取自菌株 Philadelphia 的 PAI-1（致病岛）的已发表数据。设计了特异性引物，并在凝胶基础聚合酶链反应（PCR）试验中使用。mip 基因的 PCR 扩增用作对照。终点是在溴化乙锭染色凝胶上是否存在 PCR 产物。在本研究中，区分指数略低于 SBT（0.87 对 0.97）。尽管如此，所获得的结果表明，这种简单快速的分型检测可能有助于嗜肺军团菌菌株的流行病学特征描述。
