[基于序列的广州环境嗜肺军团菌分离株分型]
[Sequence-based typing of enviromental Legionella pneumophila isolates in Guangzhou].
作者信息
Zhang Ying, Qu Pinghua, Zhang Jian, Chen Shouyi
机构信息
Guangzhou Center for Disease Control and Prevention, Guangzhou 510080, China.
出版信息
Wei Sheng Wu Xue Bao. 2011 Mar;51(3):320-5.
OBJECTIVE
To characterize the genes of Legionella pneumophila isolated from different water source in Guangzhou from 2006 to 2009. To genotype the strains by using sequence-based typing (SBT) scheme.
METHODS
In total 44 L. pneumophila strains were identified by SBT with 7 diversifying genes of flaA, asd, mip, pilE, mompS, proA and neuA. Analysis of the amplicons sequence was taken in the European Working Group for Legionella Infections (EWGLI) international SBT database to obtain the allelic profiles and sequence types (STs). Serogroups were typed by latex agglutination test.
RESULTS
Data from SBT revealed a high diversity among the strains and ST01 accounts for 30% (13/ 44). Fifteen new STs were discovered from 20 STs and 2 of them were newly assigned (ST887 and ST888) by EWGLI. SBT Phylogenetic tree was generated by SplitsTree and BURST programs.
CONCLUSION
High diversity and specificity were observed of the L. pneumophila strains in Guangzhou. SBT is useful for L. pneumophila genomic study and epidemiological surveillance.
目的
对2006年至2009年从广州不同水源分离出的嗜肺军团菌基因进行特征分析。采用基于序列的分型（SBT）方案对菌株进行基因分型。
方法
用SBT法对44株嗜肺军团菌进行鉴定，所用的7个可变基因分别为flaA、asd、mip、pilE、mompS、proA和neuA。在欧洲军团菌感染工作组（EWGLI）国际SBT数据库中对扩增子序列进行分析，以获得等位基因谱和序列类型（STs）。通过乳胶凝集试验对血清群进行分型。
结果
SBT数据显示菌株间存在高度多样性，ST01占30%（13/44）。从20种STs中发现了15种新的STs，其中2种被EWGLI新指定（ST887和ST888）。通过SplitsTree和BURST程序生成SBT系统发育树。
结论
广州嗜肺军团菌菌株具有高度多样性和特异性。SBT对嗜肺军团菌基因组研究和流行病学监测有用。
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