Wieloch Maria, Swiatkowska Magdalena, Libiszewski Michał, Hedayati Masoud, Drozda Rafał, Kołomecki Krzysztof
Szpital MSWiA w Łodzi, Oddział Chirurgii Ogólnej.
Pol Merkur Lekarski. 2009 Dec;27(162):466-9.
Angiogenesis is a process of new blood vessels creating based on existing already vascularisation. This composed and multistage mechanism, which is responsible for growth of the tissues and organs, plays a crucial role in neoplasia. The exact role of particular cells, cytokines and extracellular matrix in cancerogenesis is still discussing. An identification of it is the key to searching the antiangiogenic substances breaking simultaneously neoplastic proliferation. THE AIM OF THE STUDY was to compare the angiopoietin-1(Ang-1), angiopoietin-2 (Ang-2) and Tie-2 receptor (rec. Tie-2) concentrations in the serum of colorectal cancer patients with concentrations of these factors in the serum of healthy people. Moreover the aim of the study was to assess the correlation between the concentrations of these factors and stage of clinical neoplasm progression.
The study included 35 patients hospitalized in the Department of General Surgery due to confirmed by histopatological examination colorectal cancer, between 2006 to 2007. The group of examined patients consisted of 9 women and 26 men with ages between 39 to 81 (average 67.20+/-8.85). The control group consisted of 11 healthy people. The examinations relied on the 10 ml of blood sampling from all the patients, in fasting state, one day before the operation. 10 ml of blood, in the healthy group was also sampled in fasting state. Afterwards all blood samples, 30 minutes after collecting, were centrifuged with velocity 6000 rotation per minute. The gaining serum was freezed in temperature -80 degrees C. The concentrations of Ang-1, Ang-2 and rec.Tie-2 were measured with ELISA method.
Among examined patients with colorectal cancer, the statistically significant, higher concentration of Ang-2 in comparison with control group was revealed (6239.7+/-3482.3 pg/ml vs. 2954.9+/-9624.2 pg/ml). The concentration of Ang-2 among the patients from III and IV group according to the clinical and pathomorfological progression classification was statistically significant, higher than in I and II group of the patients (III: 8668,8+/-4938.57 pg/ml; IV: 9002.1+/-5380.92 pg/ml vs. I: 4672.3+/-1808.7 pg/ml; II: 5284.8+/-3204.2 pg/ml). The concentration of Ang-1 and rec. Tie-2 in blood of all the patients with colorectal cancer didn't statistically differ from the control group (Ang-1: 42339.9+/-20197.2 pg/ml vs. 48976.4+/-2284.5 pg/ml; rec.Tie-2; 14.4+/-5.1 vs. 16.8+/-6.2 ng/ml).
The concentration of Ang-2 in the colorectal patients was statistically higher than in the control group. The increase concentration of Ang-2 correlated with the stage of colorectal cancer's clinical progression. The higher concentration of Ang-2 may eventually become a helpful marker in diagnostic and assessment of colorectal cancer's clinical progression stage.
血管生成是基于已有的血管化过程产生新血管的过程。这种复杂的多阶段机制负责组织和器官的生长，在肿瘤形成中起关键作用。特定细胞、细胞因子和细胞外基质在癌症发生的确切作用仍在讨论中。对其进行鉴定是寻找能同时阻断肿瘤增殖的抗血管生成物质的关键。本研究的目的是比较结直肠癌患者血清中血管生成素-1（Ang-1）、血管生成素-2（Ang-2）和Tie-2受体（rec.Tie-2）的浓度与健康人血清中这些因子的浓度。此外，本研究的目的是评估这些因子浓度与临床肿瘤进展阶段之间的相关性。
本研究纳入了2006年至2007年间因组织病理学检查确诊为结直肠癌而入住普通外科的35例患者。受检患者组包括9名女性和26名男性，年龄在39至81岁之间（平均67.20±8.85）。对照组由11名健康人组成。检查依赖于在手术前一天对所有患者空腹采集10毫升血液。健康组也在空腹状态下采集10毫升血液。之后，所有血液样本在采集后30分钟以每分钟6000转的速度离心。获得的血清在-80摄氏度下冷冻。采用ELISA法测定Ang-1、Ang-2和rec.Tie-2的浓度。
在接受检查的结直肠癌患者中，与对照组相比，Ang-2的浓度在统计学上显著更高（6239.7±3482.3皮克/毫升对2954.9±9624.2皮克/毫升）。根据临床和病理形态学进展分类，III组和IV组患者中Ang-2的浓度在统计学上显著高于I组和II组患者（III组：8668.8±4938.57皮克/毫升；IV组：9002.1±5380.92皮克/毫升对I组：4672.3±1808.7皮克/毫升；II组：5284.8±3204.2皮克/毫升）。所有结直肠癌患者血液中Ang-1和rec.Tie-2的浓度与对照组在统计学上无差异（Ang-1：42339.9±20197.2皮克/毫升对48976.4±2284.5皮克/毫升；rec.Tie-2：14.4±5.1对16.8±6.2纳克/毫升）。
结直肠癌患者中Ang-2的浓度在统计学上高于对照组。Ang-2浓度的升高与结直肠癌的临床进展阶段相关。较高的Ang-2浓度最终可能成为结直肠癌诊断和临床进展阶段评估的有用标志物。
