姜黄素抑制人胰腺癌细胞系中组成性 STAT3 磷酸化和 survivin/BIRC5 基因表达下调。

Curcumin inhibits constitutive STAT3 phosphorylation in human pancreatic cancer cell lines and downregulation of survivin/BIRC5 gene expression.

机构信息

Department of Internal Medicine II, Hematology and Oncology, University Hospital, Johann Wolfgang Goethe University, Frankfurt, Germany.

出版信息

Cancer Invest. 2010 Feb;28(2):166-71. doi: 10.3109/07357900903287006.

DOI:10.3109/07357900903287006

PMID:20121547

Abstract

The purpose of this study was to determine the effect of curcumin on Survivin/BIRC5 and on the role of signal transducer and activator of transcription 3 (STAT3) activation in Survivin/ BIRC5. We incubated two pancreatic cancer cell lines with different amounts of curcumin. This resulted in a downregulation of proliferation in all cell lines tested. The expression of Survivin/BIRC5 on mRNA and protein level was significantly downregulated and the phosphorylation of STAT3 was blocked. Treatment of pancreatic cancer cells with curcumin resulted in an induction of apoptosis. The results indicate that curcumin inhibits several key factors in cancer cellular pathways and may be of interest in pancreatic cancer.

摘要

本研究旨在确定姜黄素对 Survivin/BIRC5 的影响，以及信号转导和转录激活因子 3（STAT3）激活在 Survivin/BIRC5 中的作用。我们用不同剂量的姜黄素孵育两种胰腺癌细胞系。结果显示，所有检测的细胞系的增殖均受到下调。 Survivin/BIRC5 的表达在 mRNA 和蛋白水平上均显著下调，STAT3 的磷酸化被阻断。用姜黄素处理胰腺癌细胞导致细胞凋亡的诱导。结果表明，姜黄素抑制了癌症细胞通路中的几个关键因素，可能对胰腺癌有一定的研究价值。