Zhang Jing, Kang Dianmin, Fu Jihua, Sun Xiaoguang, Lin Bin, Bi Zhenqiang, Nkengasong John N, Yang Chunfu
Institute of AIDS/HIV Control and Prevention, Shandong Center for Disease Control and Prevention, China.
AIDS Res Hum Retroviruses. 2010 Jan;26(1):99-103. doi: 10.1089/aid.2009.0184.
A survey to measure transmitted HIV-1 drug resistance (DR) was conducted in 2006 following the World Health Organization threshold survey protocol. Dried blood spots (DBS) were prepared from 53 newly HIV-1-diagnosed patients. Protease and reverse transcriptase (RT) gene regions were sequenced using a broadly sensitive genotyping assay and analyses to identify DR mutations and determine phylogeny of the HIV-1 strains were conducted. Forty-six of the 47 successfully genotyped DBS had no transmitted DR mutations; one had an NNRTI mutation (K101E) in the RT region. Phylogenetic analyses revealed 21 (44.7%) were CRF01_AE, 9 (19.1%) B, 6 (12.8%) CRF07_BC, 3 (6.4%) each of CRF08_BC and C, and 2 (4.3%) B/C unique recombinant forms (URF). The remaining three were one each of A/B, A/C, and unclassifiable. Our analyses indicate that the prevalence of transmitted DR in this population is low and the HIV-1 epidemic in the area was characterized by multiple subtypes and recombinant forms.
2006年，按照世界卫生组织阈值调查方案开展了一项测量传播的HIV-1耐药性（DR）的调查。从53例新诊断出HIV-1的患者中制备了干血斑（DBS）。使用广泛敏感的基因分型检测对蛋白酶和逆转录酶（RT）基因区域进行测序，并进行分析以鉴定DR突变并确定HIV-1毒株的系统发育。在47例成功进行基因分型的DBS中，有46例没有传播的DR突变；1例在RT区域有一个非核苷类逆转录酶抑制剂（NNRTI）突变（K101E）。系统发育分析显示，21例（44.7%）为CRF01_AE，9例（19.1%）为B型，6例（12.8%）为CRF07_BC，CRF08_BC和C型各3例（6.4%），2例（4.3%）为B/C独特重组形式（URF）。其余3例分别为A/B、A/C和无法分类的各1例。我们的分析表明，该人群中传播的DR流行率较低，该地区的HIV-1流行以多种亚型和重组形式为特征。
