National Food Institute, The Technical University of Denmark, Søborg, Denmark.
Euro Surveill. 2010 Jan 28;15(4):19471.
Phage typing has for decades been useful as a phenotypical, definitive method for epidemiological characterisation of Salmonella Typhimurium. The system recommended by the World Health Organization (WHO) Collaborative Centre for phage typing of Salmonella has, however, become rather complex, and the present study illustrates the challenges of sufficient standardisation of the interpretation of lysis results to make sure that the same strain is assigned to the same phage type in different laboratories. Even though molecular typing methods will replace phenotypic characterisation methods in the future, it is our opinion that phage typing will remain for some time a useful tool to strengthen global Salmonella surveillance. Therefore, improved standardisation and quality assurance is essential to obtain a robust and harmonized method that allows comparison of results between laboratories.
噬菌体分型几十年来一直是一种有用的表型方法，可用于对肠炎沙门氏菌进行流行病学特征描述。然而，世界卫生组织（WHO）推荐的噬菌体分型沙门氏菌协作中心的系统变得相当复杂，本研究说明了充分标准化裂解结果解释的挑战，以确保在不同实验室中对同一菌株进行相同的噬菌体分型。尽管分子分型方法将在未来取代表型特征描述方法，但我们认为噬菌体分型在一段时间内仍将是加强全球沙门氏菌监测的有用工具。因此，改进标准化和质量保证对于获得稳健且协调的方法以实现实验室间结果比较至关重要。
