Endocrine Research Laboratories, Medizinische Klinik-Innenstadt, Ludwig-Maximilians University, Munich, Germany.
Endocrinol Metab Clin North Am. 2010 Mar;39(1):25-32, vii. doi: 10.1016/j.ecl.2009.10.006.
Catching athletes abusing human growth hormone (GH) by official antidoping tests is challenging because of specific properties of the hormone. Furthermore, the chemical structure of recombinant GH (rGH) is identical to that of the main GH isoform secreted by the pituitary, making it difficult to discriminate between endogenous and injected GH molecules by biochemical tests. The approaches developed to solve the problem include the "marker approach," which measures changes in concentration of GH-dependent proteins that are inappropriately elevated after rGH injection, and the "isoform approach," which detects changes in the spectrum of circulating GH isoforms after administration of rGH. A more widespread use of these tests in out-of-competition controls will enhance the likelihood to detect GH doping.
利用官方反兴奋剂检测来捕捉运动员滥用人生长激素（GH）是具有挑战性的，因为激素具有特定的属性。此外，重组人生长激素（rGH）的化学结构与垂体分泌的主要 GH 同工型完全相同，这使得通过生化检测很难区分内源性和注射的 GH 分子。为解决这个问题而开发的方法包括“标记物方法”，该方法测量 rGH 注射后不适当升高的 GH 依赖性蛋白浓度的变化，以及“同工型方法”，该方法检测 rGH 给药后循环 GH 同工型谱的变化。在非比赛控制中更广泛地使用这些检测方法将提高检测 GH 兴奋剂的可能性。
