Kenya Medical Research Institute, Centre for Geographic Medicine Research Coast, Entomology, P.O. Box 428, 80108 Kilifi, Kenya.
Acta Trop. 2010 May;114(2):103-8. doi: 10.1016/j.actatropica.2010.01.011. Epub 2010 Feb 1.
In the tropics, Anopheles mosquito abundance is greatest during the wet season and decline significantly during the dry season as larval habitats shrink. Population size fluctuations between wet and dry seasons may lead to variation in distribution of specific alleles within natural Anopheles populations, and a possible effect on the population genetic structure. We used 11 microsatellite markers to examine the effect of seasonality on population genetic structure of Anopheles gambiae s.s. at two sites along the Kenyan coast. All loci were highly polymorphic with the total number of alleles for pooled samples ranging from 7 (locus ND36) to 21 (locus AG2H46). Significant estimates of genetic differentiation between sites and seasons were observed suggesting the existence of spatio-temporal subpopulation structuring. Genetic bottleneck analysis showed no indication of excess heterozygosity in any of the populations. These findings suggest that along the Kenyan coast, seasonality and site specific ecological factors can alter the genetic structure of A. gambiae s.s. populations.
在热带地区，疟蚊的数量在雨季最大，随着幼虫栖息地的缩小，在旱季会显著减少。干湿季之间的种群数量波动可能导致自然疟蚊种群中特定等位基因的分布发生变化，并可能对种群遗传结构产生影响。我们使用 11 个微卫星标记来研究季节性对肯尼亚沿海两个地点冈比亚按蚊种群遗传结构的影响。所有的位点都具有高度多态性， pooled 样本的等位基因总数从 7 个（ND36 位点）到 21 个（AG2H46 位点）不等。观察到地点和季节之间存在显著的遗传分化估计值，表明存在时空亚种群结构。遗传瓶颈分析表明，在任何一个种群中都没有多余的杂合子。这些发现表明，在肯尼亚沿海地区，季节性和特定地点的生态因素可以改变冈比亚按蚊种群的遗传结构。
