Advanced Biotechnology and Breeding Centre, Biological Research Division, Malaysian Palm Oil Board, P.O. Box 10620, 50720 Kuala Lumpur, Malaysia.
N Biotechnol. 2010 Sep 30;27(4):289-99. doi: 10.1016/j.nbt.2010.01.337. Epub 2010 Feb 1.
The ubiquitin extension protein (uep1) gene was identified as a constitutively expressed gene in oil palm. We have isolated and characterized the 5' region of the oil palm uep1 gene, which contains an 828 bp sequence upstream of the uep1 translational start site. Construction of a pUEP1 transformation vector, which contains gusA reporter gene under the control of uep1 promoter, was carried out for functional analysis of the promoter through transient expression studies. It was found that the 5' region of uep1 functions as a constitutive promoter in oil palm and could drive GUS expression in all tissues tested, including embryogenic calli, embryoid, immature embryo, young leaflet from mature palm, green leaf, mesocarp and meristematic tissues (shoot tip). This promoter could also be used in dicot systems as it was demonstrated to be capable of driving gusA gene expression in tobacco.
泛素延伸蛋白 (uep1) 基因被鉴定为油棕中组成型表达的基因。我们已经分离并鉴定了油棕 uep1 基因的 5' 区域，该区域包含 uep1 翻译起始位点上游的 828bp 序列。构建了 pUEP1 转化载体，其中包含在 uep1 启动子控制下的 gusA 报告基因，用于通过瞬时表达研究对启动子的功能进行分析。结果发现，uep1 的 5' 区域在油棕中作为组成型启动子发挥作用，可以驱动所有测试组织中的 GUS 表达，包括胚性愈伤组织、胚状体、未成熟胚胎、成熟棕榈的小叶、绿叶、中果皮和分生组织（茎尖）。该启动子也可用于双子叶系统，因为它能够在烟草中驱动 gusA 基因的表达。
