Graduate School of Environmental and Human Sciences, Meijo University, Nagoya 468-8502, Japan.
Tree Physiol. 2011 Apr;31(4):462-8. doi: 10.1093/treephys/tpr017. Epub 2011 Apr 7.
Oil production from oil palm is adversely affected by drought and salt. Under drought and salt stress, proline content increases in oil palm; the mechanism for this is unknown. Here, an 8319-nucleotide sequence including cDNA, genomic DNA and the promoter region of proline transporter gene from oil palm Elaeis guineensis was determined. The transporter gene exhibited high similarity to Bet/ProT genes from several plants, but the highest homology was found with rice ProT1. The exon-intron structure of genomic DNA was unique, and numerous stress-response cis-elements were found in the promoter region. Expression of cDNA EgProT1 in Escherichia coli mutant exhibited uptake activities for glycinebetaine and choline as well as proline. Under salt-stressed conditions, exogenously applied glycinebetaine was taken up into the root more rapidly than the control. These data indicate that oil palm has a unique Pro/T1 gene. Nucleotide sequence data for the cDNA and genomic DNA of proline transporter gene from Elaeis guineensis are available in the DDJB database under accession numbers AB597035 and AB597036, respectively.
油棕的产油量受到干旱和盐分的不利影响。在干旱和盐胁迫下，油棕中的脯氨酸含量增加；其机制尚不清楚。在这里，确定了油棕（Elaeis guineensis）脯氨酸转运基因的 cDNA、基因组 DNA 和启动子区域的 8319 个核苷酸序列。该转运基因与几种植物的 Bet/ProT 基因具有高度相似性，但与水稻 ProT1 的同源性最高。基因组 DNA 的exon-intron 结构是独特的，并且在启动子区域发现了许多应激反应顺式元件。cDNA EgProT1 在大肠杆菌突变体中的表达表现出对甘氨酸甜菜碱和胆碱以及脯氨酸的摄取活性。在盐胁迫条件下，外源施加的甘氨酸甜菜碱比对照更快地被吸收到根部。这些数据表明油棕具有独特的 Pro/T1 基因。油棕脯氨酸转运基因的 cDNA 和基因组 DNA 的核苷酸序列数据已在 DDJB 数据库中分别以 AB597035 和 AB597036 的登录号提供。
