McIntosh A R, Stillman M J
Biochem J. 1977 Oct 1;167(1):31-7. doi: 10.1042/bj1670031.
Strong electron-paramagnetic-resonance signals in the g = 2.00 region were detected after irradiation of horseradish peroxidase Compound I at temperatures of 10 and 100 K. These signals establish the presence of new free-radical species in the peroxidase system. The new species are interpreted in terms of a haem-photosensitized oxidation of the protein's peptide groups close to the Compound I radical site. On warming to room temperature, the radicals decayed irreversibly to a species having a weak asymmetric electron-paramagnetic-resonance signal at 100 K, which could still be observed after incubation at room temperature for more than 1 h.
在10 K和100 K的温度下照射辣根过氧化物酶化合物I后，在g = 2.00区域检测到强电子顺磁共振信号。这些信号表明过氧化物酶系统中存在新的自由基物种。这些新物种被解释为靠近化合物I自由基位点的蛋白质肽基团的血红素光敏氧化。升温至室温后，自由基不可逆地衰减为在100 K时具有弱不对称电子顺磁共振信号的物种，在室温下孵育超过1小时后仍可观察到该信号。
