通过还原型烟酰胺腺嘌呤二核苷酸和吩嗪硫酸甲酯对芳香族化合物进行羟基化反应需要过氧化氢和羟基自由基，但不需要超氧化物。
Hydroxylation of aromatic compounds by reduced nicotinamide-adenine dinucleotide and phenazine methosulphate requires hydrogen peroxide and hydroxyl radicals, but not superoxide.
作者信息
Halliwell B
出版信息
Biochem J. 1977 Oct 1;167(1):317-20. doi: 10.1042/bj1670317.
Abstract
- A mixture of NADH and phenazine methosulphate hydroxylates aromatic compounds at acidic pH values. 2. Hydroxylation is inhibited by catalase and by scavengers of the hydroxyl radical (-OH) but not by superoxide dismutase. 3. It is concluded that neither O2 leads to nor HO2- is sufficiently reactive to hydroxylate aromatic rings.
摘要
- 烟酰胺腺嘌呤二核苷酸（NADH）和硫酸甲基吩嗪的混合物在酸性pH值下可使芳香族化合物羟基化。2. 过氧化氢酶和羟基自由基（-OH）清除剂可抑制羟基化反应，但超氧化物歧化酶不能。3. 得出的结论是，超氧阴离子（O2-）和超氧羟自由基（HO2-）的反应活性均不足以使芳香环羟基化。
相似文献
1
Hydroxylation of aromatic compounds by reduced nicotinamide-adenine dinucleotide and phenazine methosulphate requires hydrogen peroxide and hydroxyl radicals, but not superoxide.通过还原型烟酰胺腺嘌呤二核苷酸和吩嗪硫酸甲酯对芳香族化合物进行羟基化反应需要过氧化氢和羟基自由基，但不需要超氧化物。
Biochem J. 1977 Oct 1;167(1):317-20. doi: 10.1042/bj1670317.
2
Free radical production from the aerobic oxidation of reduced pyridine nucleotides catalysed by phenazine derivatives.吩嗪衍生物催化还原型吡啶核苷酸的有氧氧化产生自由基。
Biochim Biophys Acta. 1983 Sep 30;724(3):456-64. doi: 10.1016/0005-2728(83)90106-8.
3
On the mechanism of production of superoxide radical by reaction mixtures containing NADH, phenazine methosulfate, and nitroblue tetrazolium.关于含有NADH、硫酸吩嗪甲酯和硝基蓝四氮唑的反应混合物产生超氧自由基的机制
Arch Biochem Biophys. 1984 Jan;228(1):155-8. doi: 10.1016/0003-9861(84)90056-0.
4
A chemiluminescence (CL) of phenazine methosulfate (PMS) in the presence of hydrogen peroxide (HOOH) induced by reductants including reduced nicotinamide adenine dinucleotide (NADH) and ascorbic acid (AA).在包括还原型烟酰胺腺嘌呤二核苷酸（NADH）和抗坏血酸（AA）在内的还原剂存在下，吩嗪硫酸甲酯（PMS）在过氧化氢（HOOH）存在时会产生化学发光（CL）。
Biochem Biophys Res Commun. 1963 Mar 5;10:390-5. doi: 10.1016/0006-291x(63)90543-6.
5
Studies on the reduction of nitroblue tetrazolium chloride mediated through the action of NADH and phenazine methosulphate.关于通过NADH和吩嗪硫酸甲酯的作用介导氯化硝基四氮唑蓝还原的研究。
Chem Biol Interact. 1978 Dec;23(3):281-91. doi: 10.1016/0009-2797(78)90090-x.
6
Studies on the phenazine methosulphate--tetrazolium salt capture reaction in NAD(P)+-dependent dehydrogenase cytochemistry. III. The role of superoxide in tetrazolium reduction.关于吩嗪硫酸甲酯 - 四唑盐捕获反应在NAD(P)+依赖的脱氢酶细胞化学中的研究。III. 超氧化物在四唑还原中的作用。
Histochem J. 1983 Oct;15(10):977-86. doi: 10.1007/BF01002493.
7
Inhibition by superoxide dismutase and catalase of the damage of isolated Leishmania mexicana amazonensis by phenazine methosulfate.超氧化物歧化酶和过氧化氢酶对硫酸吩嗪所致的离体亚马逊利什曼原虫损伤的抑制作用
Mol Biochem Parasitol. 1984 Mar;10(3):297-303. doi: 10.1016/0166-6851(84)90028-8.
8
TITRATION OF CYTOCHROME C OXIDASE WITH NADH AND PHENAZINE METHOSULPHATE.用还原型辅酶Ⅰ和硫酸吩嗪对细胞色素C氧化酶进行滴定
Biochim Biophys Acta. 1963 Aug 6;73:663-5. doi: 10.1016/0006-3002(63)90342-1.
9
The involvement of superoxide anions in the nitro blue tetrazolium chloride reduction mediated by NADH and phenazine methosulfate.超氧阴离子在由NADH和硫酸吩嗪介导的氯化硝基四氮唑蓝还原反应中的作用。
Anal Biochem. 1989 Jan;176(1):170-4. doi: 10.1016/0003-2697(89)90288-1.
10
Reactive oxygen species involved in phenazine-methosulfate-induced rat lens opacification. An experimental model of cataract.参与吩嗪硫酸甲酯诱导大鼠晶状体混浊的活性氧。白内障的实验模型。
Ophthalmic Res. 1994;26(1):41-50. doi: 10.1159/000267373.
本文引用的文献
1
Superoxide dismutase. An enzymic function for erythrocuprein (hemocuprein).超氧化物歧化酶。红细胞铜蛋白（血铜蛋白）的酶功能。
J Biol Chem. 1969 Nov 25;244(22):6049-55.
2
The occurrence of superoxide anion in the reaction of reduced phenazine methosulfate and molecular oxygen.还原型硫酸吩嗪与分子氧反应中超氧阴离子的产生。
Biochem Biophys Res Commun. 1972 Jan 31;46(2):849-54. doi: 10.1016/s0006-291x(72)80218-3.
3
Mechanism of aromatic hydroxylation. Properties of a model for pyridine nucleotide-dependent flavoprotein hydroxylases.芳香族羟基化作用的机制。吡啶核苷酸依赖性黄素蛋白羟化酶模型的性质。
Arch Biochem Biophys. 1974 Dec;165(2):478-84. doi: 10.1016/0003-9861(74)90273-2.
4
On the development of a well-defined source of superoxide ion for studies with biological systems.关于开发一种用于生物系统研究的明确超氧阴离子源。
FEBS Lett. 1974 Feb 1;39(1):79-82. doi: 10.1016/0014-5793(74)80021-9.
5
Superoxide dismutase: a contaminant of bovine catalase.超氧化物歧化酶：牛过氧化氢酶的一种污染物。
Biochem J. 1973 Oct;135(2):379-81. doi: 10.1042/bj1350379.
6
Mechanism of hydroxylation of aromatic compounds: evidence for the involvement of superoxide anion in a model system.芳香族化合物的羟基化机制：超氧阴离子参与模型系统的证据。
Biochem Biophys Res Commun. 1972 Sep 5;48(5):1049-54. doi: 10.1016/0006-291x(72)90814-5.
7
Superoxide dismutases.超氧化物歧化酶
Annu Rev Biochem. 1975;44:147-59. doi: 10.1146/annurev.bi.44.070175.001051.
8
Hydroxylation of p-coumaric acid by horseradish peroxidase. The role of superoxide and hydroxyl radicals.辣根过氧化物酶催化对香豆酸的羟基化反应。超氧自由基和羟基自由基的作用。
Biochem J. 1976 Mar 1;153(3):513-8. doi: 10.1042/bj1530513a.
9
Oxidation of manganous pyrophosphate by superoxide radicals and illuminated spinach chloroplasts.超氧自由基和光照下的菠菜叶绿体对焦磷酸锰的氧化作用。
Arch Biochem Biophys. 1976 Jun;174(2):454-62. doi: 10.1016/0003-9861(76)90373-8.
10
Mechanism of aromatic hydroxylation : involvement of perhydroxyl radicals and semiquinones in hydroxylation of aromatic amino acids by a model system.芳香族羟基化的机制：过羟基自由基和半醌在模型系统中对芳香族氨基酸羟基化的参与
Indian J Biochem Biophys. 1975 Jun;12(2):163-7.
Zotero 插件