USC Epigenome Center, University of Southern California, Keck School of Medicine, Los Angeles, 90089‑9601, USA.
Nat Rev Genet. 2010 Mar;11(3):191-203. doi: 10.1038/nrg2732.
Methylation of cytosine bases in DNA provides a layer of epigenetic control in many eukaryotes that has important implications for normal biology and disease. Therefore, profiling DNA methylation across the genome is vital to understanding the influence of epigenetics. There has been a revolution in DNA methylation analysis technology over the past decade: analyses that previously were restricted to specific loci can now be performed on a genome-scale and entire methylomes can be characterized at single-base-pair resolution. However, there is such a diversity of DNA methylation profiling techniques that it can be challenging to select one. This Review discusses the different approaches and their relative merits and introduces considerations for data analysis.
DNA 中胞嘧啶碱基的甲基化在许多真核生物中提供了一层表观遗传控制，这对正常生物学和疾病都有重要意义。因此，对基因组中 DNA 甲基化进行分析对于理解表观遗传学的影响至关重要。在过去的十年中，DNA 甲基化分析技术发生了革命性的变化：以前仅限于特定基因座的分析现在可以在全基因组范围内进行，并且可以在单个碱基对分辨率下对整个甲基组进行特征描述。然而，DNA 甲基化分析技术种类繁多，因此选择一种技术可能具有挑战性。本文综述了不同的方法及其相对优缺点，并介绍了数据分析的注意事项。
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