Reinders Jon, Delucinge Vivier Céline, Theiler Grégory, Chollet Didier, Descombes Patrick, Paszkowski Jerzy
Laboratory of Plant Genetics, Department of Plant Biology, University of Geneva, CH-1211 Geneva 4, Switzerland.
Genome Res. 2008 Mar;18(3):469-76. doi: 10.1101/gr.7073008. Epub 2008 Jan 24.
Methylation of cytosines ((m)C) is essential for epigenetic gene regulation in plants and mammals. Aberrant (m)C patterns are associated with heritable developmental abnormalities in plants and with cancer in mammals. We have developed a genome-wide DNA methylation profiling technology employing a novel amplification step for DNA subjected to bisulfite-mediated cytosine conversion. The methylation patterns detected are not only consistent with previous results obtained with (m)C immunoprecipitation (mCIP) techniques, but also demonstrated improved resolution and sensitivity. The technology, named BiMP (for Bisulfite Methylation Profiling), is more cost-effective than mCIP and requires as little as 100 ng of Arabidopsis DNA.
胞嘧啶甲基化((m)C)对于植物和哺乳动物的表观遗传基因调控至关重要。异常的(m)C模式与植物中可遗传的发育异常以及哺乳动物中的癌症相关。我们开发了一种全基因组DNA甲基化分析技术,该技术采用了一个新的扩增步骤来处理经亚硫酸氢盐介导的胞嘧啶转化后的DNA。检测到的甲基化模式不仅与先前通过(m)C免疫沉淀(mCIP)技术获得的结果一致,而且分辨率和灵敏度也有所提高。这项名为BiMP(亚硫酸氢盐甲基化分析)的技术比mCIP更具成本效益,并且仅需100 ng拟南芥DNA。