Banerjee Indranil, Mishra Debasish, Maiti Tapas K
Department of Biotechnology, Indian Institute of Technology, Kharagpur, West Bengal 721302, India.
Int J Biomater. 2009;2009:143659. doi: 10.1155/2009/143659. Epub 2009 Mar 30.
Freeze drying is one of the popular methods of fabrication for poly(lactide-co-glycolide) (PLGA) microspheres incorporated polymer scaffolds. However, the consequence of microspheres incorporation on physical and biological properties of scaffold has not been studied yet. In this study, attempt has been made to characterize the effect of PLGA microsphere incorporation on the physical properties of freeze-dried gelatin scaffold and its influence on cytocompatibility. Scaffolds loaded with varying amount of PLGA microspheres (10%, 1%, 0.1% w/w) were subjected to microarchitecture analysis, swelling, porosity, mechanical properties, biodegradation, cell adhesion, and cell proliferation studies. Results revealed that an increase in percentage loading of microspheres reduced the pore size and uniformity of the pore structure. Moreover, loading of PLGA microspheres up to 1% w/w significantly increased porosity, swelling, and mechanical properties of the scaffold but variations were not proportional for 10% w/w loading. Results also showed that PLGA microspheres have no significant effect on cell adhesion but influenced the growth kinetics.
冷冻干燥是制备聚（丙交酯-乙交酯）（PLGA）微球复合聚合物支架的常用方法之一。然而，微球复合对支架物理和生物学性能的影响尚未得到研究。在本研究中，试图表征PLGA微球复合对冷冻干燥明胶支架物理性能的影响及其对细胞相容性的影响。对负载不同量PLGA微球（10%、1%、0.1% w/w）的支架进行微观结构分析、溶胀、孔隙率、力学性能、生物降解、细胞黏附和细胞增殖研究。结果表明，微球负载百分比的增加会降低孔径和孔结构的均匀性。此外，PLGA微球负载量高达1% w/w时，支架的孔隙率、溶胀和力学性能显著增加，但对于10% w/w的负载量，变化不成比例。结果还表明，PLGA微球对细胞黏附没有显著影响，但影响生长动力学。
