Department of Environmental Pollution Biophysics, University of Łódź, Banacha 12/16 St., 90-237 Łódź, Poland.
Arch Toxicol. 2010 May;84(5):379-87. doi: 10.1007/s00204-010-0515-y. Epub 2010 Feb 2.
In this study, the effect of environmental toxins such as pentachlorophenol (PCP), tetrachlorocatechol (TeCC) and tetrachloroguaiacol (TeCG) on human peripheral blood lymphocytes was investigated. All the compounds studied increased the size and granularity of the lymphocytes in the concentrations range from 5 to 600 ppm. The PCP caused the strongest increase in the size of the cells, whereas lymphocytes granularity was more strongly increased by TeCC and PCP than by TeCG. The PCP and its derivatives in the concentrations range from 1 to 125 ppm significantly depleted ATP level. It was also observed that PCP most strongly decreased ATP content at its highest concentration of 125 ppm. Moreover, PCP caused the highest loss of lymphocytes viability in the concentrations range from 125 to 600 ppm. The TeCC in the concentrations of 1 and 5 ppm significantly increased the level of strand breaks in DNA, whereas lower damage was noted for PCP, and particularly for TeCG. The increase in carbonyl groups content was more strongly induced by TeCG and TeCC than by PCP in the concentrations range from 0.04 to 1 ppm; however, in a concentration of 5 ppm, all the compounds studied increased this parameter to a similar degree. DNA and protein damage was the most probably induced by free radical formation, as it was observed that all the compounds examined, and TeCC, in particular, were able of oxidize a fluorescent probe 6-carboxy-2',7'-dichlorodihydrofluorescein in the concentrations range from 0.01 to 1 ppm.
在这项研究中，研究了环境毒素如五氯苯酚（PCP）、四氯邻苯二酚（TeCC）和四氯愈创木酚（TeCG）对人外周血淋巴细胞的影响。所有研究的化合物在 5 至 600 ppm 的浓度范围内增加了淋巴细胞的大小和颗粒度。PCP 导致细胞大小的增加最强，而 TeCC 和 PCP 比 TeCG 更强烈地增加了淋巴细胞的颗粒度。PCP 及其衍生物在 1 至 125 ppm 的浓度范围内显著降低了 ATP 水平。还观察到 PCP 在其最高浓度 125 ppm 时最强地降低了 ATP 含量。此外，PCP 在 125 至 600 ppm 的浓度范围内导致淋巴细胞活力的最高损失。TeCC 在 1 和 5 ppm 的浓度下显著增加了 DNA 链断裂的水平，而 PCP 则显示出较低的损伤，特别是 TeCG。在 0.04 至 1 ppm 的浓度范围内，羰基含量的增加比 PCP 更强烈地被 TeCG 和 TeCC 诱导；然而，在 5 ppm 的浓度下，所有研究的化合物都将该参数提高到相似的程度。DNA 和蛋白质的损伤很可能是由自由基形成引起的，因为观察到所有研究的化合物，特别是 TeCC，能够在 0.01 至 1 ppm 的浓度范围内氧化荧光探针 6-羧基-2'，7'-二氯二氢荧光素。
